我国韭菜主产区韭菜迟眼蕈蚊田间种群的抗药性监测

【目的】建立韭菜迟眼蕈蚊Bradysia odoriphaga Yang et Zhang对10种常用药剂的敏感基线,并对4省7个主要韭菜产区的田间种群进行6种常用杀虫剂的抗药性水平监测。【方法】采用胃毒触杀联合毒力法对韭菜迟眼蕈蚊3龄幼虫进行室内生物测定。【结果】建立了敏感品系对新烟碱类、有机磷类、菊酯类、昆虫生长调节剂类、吡咯类药剂的敏感基线。对7个地区的田间韭菜迟眼蕈蚊种群监测结果表明:其对有机磷类药剂均产生了抗药性,其中河南郑州种群对毒死蜱和辛硫磷产生了极高水平抗性;河南郑州种群对高效氯氰菊酯产生了中等水平抗性,其他各地区均处于敏感状态;大部分种群对新烟碱类药剂处于低等或中等水平抗性,但山东李坡种群对噻虫嗪产生了高水平抗性。【结论】本文建立的韭菜迟眼蕈蚊对10种杀虫剂的敏感基线及抗药性监测数据为抗性治理提供一定参考。

Monitoring the resistance of field populations of Bradysia odoriphaga Yang et Zhang in the main leek producing areas in China

Objectives] To establish susceptible toxicity baselines of Bradysia odoriphaga Yang et Zhang to 10 insecticides, and monitor the resistance of field populations of B. odoriphaga collected from 7 main leek producing areas in 4 provinces to 6 insecticides. Methods] The stomach-contact combination toxicity method was employed to determine the toxicity of insecticides to 3rd instar larvae of B. odoriphaga. Results] We established susceptible toxicity baselines to several insecticides including, Neonicotinoids, Organophosphates, Pyrethroids, Insect Growth Regulators and Azoles. The results show that resistance of field populations of B. odoriphaga to organophosphate insecticides is very common in the 7 areas surveyed, and that populations in Zhengzhou, Henan had the highest resistance to chlorpyrifos and phoxim. With the exception of the Zhengzhou Henan population, which had moderate resistance to cypermethrin, all other populations surveyed were sensitive to cypermethrin. The resistance of B. odoriphaga populations to neonicotinoids were generally low or moderate, but the population from Lipo Shandong had high resistance to thiamethoxam. Conclusion] Susceptible toxicity baselines of B. odoriphaga to 10 insecticides were established and provide a reference for managing pesticide resistance in this pest.