中华真地鳖断触角再生的研究

【目的】研究中华真地鳖Eupolyphaga sinensis(Walker)断触角再生特征及再生临界期。【方法】选取1~10龄健康的初龄若虫一条触角除基部3节外剪除,蜕皮后观察并统计触角的节数。选取生长一致健康的初龄4龄若虫,于第0~19日龄进行断触角处理,蜕皮后观察触角的再生情况和触角的节数。【结果】不同虫龄的初龄若虫断去触角蜕皮后,均能通过再生使触角的节数增加。1~10龄的若虫一条触角除基部3节外剪除,蜕皮后下一龄的触角节数分别为5、8、12、13~15、15~17、16~19、18~20、13~15、20~23、22~25、24~28节。4龄的初龄若虫一条触角分别除基部0~2、3、5、10、15、20节外剪除,蜕皮后触角的节数分别为11~12、13~15、18~21、19~25、24~27节,其中一条触角除基部20节外剪除蜕皮后基本恢复正常节数。不同日龄的4龄若虫一条触角除基部3节外剪除蜕皮后,0~8日龄的处理若虫蜕皮后触角节数为13~15节,而12日龄及以后的处理若虫蜕皮后触角节数为5~6节。4龄若虫的断触角再生临界期为9~11日龄。【结论】中华真地鳖在再生临界期内断触角后能够再生触角。

Antenna regeneration in Eupolyphaga sinensis (Blattaria：Corydiidae)

Objectives] This study was aimed to determine the characteristics and critical period of the regenerative antennae in Eupolyphaga sinensis (Walker). Methods] Healthy early 1-10th instar nymphs were amputated one of their antennae and the number of antenna segments were documented after their molting. Then 0-19 day old early 4th instar nymphs were treated, observed and counted accordingly. Results] Different instar age of nymphs of E. sinensis were able to increase the segments of their amputated antenna by regeneration. When antennal segments of 1-10th instar nymphs were amputated to only keep three basal segments, the number of antennal segments of the next instar after the nymph molted were restored as follow: 5, 8, 12, 13-15, 15-17, 16-19, 18-20, 20-23, 22-25, 24-28, respectively. When antennal segments of the 4th instar nymphs were amputated to keep 0-2,3,5,10,15 or 20 segments, the number of antennal segments of the next instar after the nymph molted were restored as follow: 11-12,13-15,15-19,18-21, 19-25 or 24-27, respectively. Among them, the antennae which were kept 20 segments basically restored to the normal number of segments after molting. Antennae of old nymphs in 4th instars were amputated to keep 3 segments during different day old: for the 0 to 8 days-old ones, the antenna segments were 13-15 after molting, and for the older than the12 days-old ones, the antenna segments were 5-6 after molting. Therefore, the critical period for regeneration antenna of 4th instar nymphs was from the 9-11 day-old. Conclusion] The amputated antennae could regenerate within the regenerative critical period of E. sinensis.