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蜜蜂褪黑素的测定方法
引用本文:李秀,秦玉川,Huang Z.Y,宋敦伦.蜜蜂褪黑素的测定方法[J].昆虫知识,2005,42(1):99-102.
作者姓名:李秀  秦玉川  Huang Z.Y  宋敦伦
作者单位:1. 中国农业大学昆虫学系,北京,100094
2. Department of Entomoloy , Michigan State University, East Lansing, MI48824, USA
基金项目:国家自然科学基金资助项目(编号:30170132)。
摘    要:在脊椎动物中,褪黑素(5-甲氧基-N-乙酰色胺,MLT)是一种由松果体分泌的具有典型光周期信号作用的神经内分泌激素。近年在昆虫头部也发现了这种化学物质。该文简述了褪黑素常见测定方法,详细介绍了褪黑素的免疫测定方法RIA(radionimmunoassay),并使用该方法初步测定了中华蜜蜂ApisceranaFabricius工蜂头部褪黑素的含量,结果显示褪黑素含量与工蜂的社会分工相关。

关 键 词:褪黑素  中华蜜蜂  放射免疫测定

A testing method for melatonin of honeybee
LI Xiu,QIN Yu-Chuan,Huang Z.Y,SUN Dun-Lun.A testing method for melatonin of honeybee[J].Entomological Knowledge,2005,42(1):99-102.
Authors:LI Xiu  QIN Yu-Chuan  Huang ZY  SUN Dun-Lun
Institution:LI Xiu1,QIN Yu-Chuan~1**,Huang Z.Y.2,SUN Dun-Lun1
Abstract:Melatonin, N-acetyl-5-methoxytryptmine, which is produced by pineal gland in the vertebrates,is a typical photoperiodic signal neuron-hormon.In recent years,this chemical is also found in many insects.This paper gave a brief review on the measurement of melationin and a detailed RIA(radioimmunoassay) assay method.Finaly,we report some preliminary results of the melationin from honeybee head (Apis cerana Fabricius),the results showed that melatonin concentrations are related to their division of labor.
Keywords:melatonin  honey bee(Apis cerana Fabricius)  RIA
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