昆虫嗅觉神经的计算机三维重建

基于激光扫描共聚焦显微镜平台的计算机三维重建在昆虫嗅觉神经研究中发挥了重要作用。对经荧光标记的神经组织采集系列光学切片并进行三维重建,在双翅目、鳞翅目、膜翅目、蜚蠊目昆虫中均有进展。触角叶是昆虫的初级嗅觉中心,触角叶的解剖学图谱是识别不同种和雌雄虫间嗅球体特定功能的先决条件。了解构成嗅觉传输途径的主要神经元的形态和空间关系是理解气味信息在中枢神经系统编码的基础。三维重建昆虫的嗅觉神经,对于探讨昆虫嗅觉在其寄主选择、觅食以及寻找配偶等行为中的作用具有非常重要的意义。

Computerized three dimensional reconstruction of the insect olfactory nerves

Based on laser scanning confocal microscope(LSCM),the computerized three-dimensional(3-D) reconstructions of the insect olfactory nerves have been applied in the insects of Orders Diptera,Lepidoptera,Hymenoptera and Blattaria.The nervous tissues labeled by fluorescence can be optically sectioned by LSCM,and a serial of the optical sections can be used for 3-D reconstruction.The map of antennal lobe,as the primary olfactory center of insects,is a prerequisite for comparison of the glomerular function among species and between male and female of cospecies.The morphology and spatial relationship of the principal neurons forming the olfactory pathways are essential for understanding the encoding procedure of odor information in the central nervous system.The insect olfactory nerves' 3-D reconstruction is of significance in exploring the role of insect olfaction in host selection,food finding and partner connection.