白纹伊蚊溴氰菊酯抗性和敏感品系羧酸酯酶性质比较

本文对白纹伊蚊Aedes albopictus溴氰菊酯抗性品系和敏感品系羧酸酯酶的生物化学性质进行了比较。白纹伊蚊抗性品系和敏感品系羧酸酯酶随底物浓度(α-乙酸萘酯或β-乙酸萘酯)的变化比活力变化趋势一致,但抗性品系对这2种底物的比活力均高于敏感品系,抗性品系羧酸酯酶的米氏常数和最大反应速度与敏感品系有显著差异。胆碱酯酶抑制剂测定结果表明,抗性品系羧酸酯酶对敌敌畏和磷酸三苯酯的敏感性高于敏感品系,对残杀威的敏感性低于敏感品系。2个品系羧酸酯酶对脱叶磷的敏感性差异不大。说明羧酸酯酶可能与白纹伊蚊对溴氰菊酯抗性有关。

Comparison of the biochemical characteristics of deltamethrin resistant and susceptible populations of Aedes albopictus

The biochemical characteristics of carboxylesterase （CarE） were investigated in deltamethrin resistant and susceptible populations of Aedes albopictus.The ability of CarE activity to hydrolyze α-naphthyl acetate （α-NA） or β-naphthyl acetate （β-NA）,dependent on substrate concentration,was similar in both populations.This specific activity was,however,significantly higher in the resistant population than in the susceptible population.CarE from the resistant population was more sensitive to DDVP（dichlorvos）and TPP（triphenyl phosphate）than that of the susceptible population.However,there was no significant difference in sensitivity to DEF（S,S,S-tributyl phosphorotrithioate）between resistant and susceptible populations.