棉铃虫β-微管蛋白基因cDNA序列的克隆与序列分析

β-微管蛋白是构成细胞骨架的重要组成性蛋白,对昆虫的蜕皮、器官形成等生长发育阶段均能产生重要影响。本文以棉铃虫Helicoverpa armigera(Hübner)3日龄成虫为材料,利用RACE末端扩增技术克隆得到棉铃虫的β-微管蛋白基因的cDNA序列。序列分析表明:棉铃虫β-微管蛋白基因的cDNA序列包含1775个碱基,包括一个1347个碱基的开放阅读框,编码448个氨基酸组成的多肽。GenBank登录号:JF767013。同源性分析表明,棉铃虫的微管蛋白基因与本研究所比对其它昆虫的β-微管蛋白基因具有高度的同源性,达到90%左右。本研究克隆得到棉铃虫的β-微管蛋白基因的cDNA序列,对进一步深入研究该基因功能有重要意义。

Molecular cloning and sequence analysis of β-tubulin in Helicoverpa armigera

β-tubulin is an important component of the cytoskeleton affecting aspects of insect development such as molting and organofaction. Total RNA was isolated from third-day adults of Helicoverpa armigera （Hübner）. A β-tubule cDNA sequence was cloned by rapid amplification of cDNA ends （RACE）. Sequence analysis indicates that the β-tubule cDNA sequence is 1 775 bp, including an open reading frame of 1 347 bp encoding a polypeptide of 448 amino acid residues. The GenBank accession number is JF767013. Phylogenetic analysis shows that the homology of amino acid sequences was about 90%, compared with other insect tubulins. The molecular cloning of the β-tubulin gene is important for further study of the function of this gene.