DPH标记法研究杀虫剂对二化螟线粒体膜流动性的影响

以DPH为荧光探剂,采用荧光偏振法研究了几种常用农药对二化螟Chilo supperssalis(Walekr)线粒体膜流动性的影响。结果表明,DPH是一种有效的荧光探剂,可以用来研究线粒体膜脂的流动性。不同种类的农药对二化螟线粒体膜的流动性都有一定的影响,但是以三氟氯氰菊酯、高效氯氰菊酯和硫丹影响较大,甲胺磷、三唑磷和克百威影响较小。三氟氯氰菊酯和高效氯氰菊酯可使膜的流动性下降,而硫丹、甲胺磷、三唑磷和克百威则使膜的流动性增强。对膜影响较大的三氟氯氰菊酯和硫丹对膜流动性的影响,还存在一定的剂量-效应关系。另外,膜的流动性受温度的影响很大,在温度分别为17、27、37℃的条件下,在药剂浓度为1×10-4mol/L时,甲胺磷在3个温度下对膜的流动性影响都很小,在误差范围内几乎没有影响;硫丹不同温度下都使膜的流动性增强,而三氟氯氰菊酯则使膜的流动性降低。

Effects of insecticides on mitochondrial membrane fluidity of Chilo suppressalis measured by a fluorescent DPH probe

The effects of some insecticides on the fluidity of the mitochondrial membrane in Chilo suppressalis （Walker） was measured using DPH as a fluorescent probe.The results show that DPH was an effective fluorescent probe with which to study mitochondrial membrane fluidity in C.suppressalis.Cyhalothrin,alphamethrin and endosulfan had a greater effect on membrane fluidity than ethamidophos,triazophos and carbafuran.Membrane fluidity was increased by Cyhalothrin and alphamethrin,but decreased by the other tested insecticides.On the other hand,membrane fluidity was also affected by temperature.The effects of cyhalothrin,endosulfan and ethamidophos on mitochondrial membrane fluidity were measured at three different temperatures （17,27 and 37℃） at insecticide concentrations of 1×10-4mol/L.Membrane fluidity was increased by endosulfan,decreased by cyhalothrin and relatively unaffected by ethamidophos,within the range of temperatures tested.