小峰熊蜂蜂王蛹期发育蛋白质组分析

为了探明小峰熊蜂Bombus hypocrita蜂王蛹期发育蛋白质表达调控方面的特点,揭示其发育的分子机理。采用双向电泳法对小峰熊蜂蜂王蛹期发育进行蛋白质组研究,结果在小峰熊蜂蜂王蛹期的白眼期(A期)、褐眼期(B期)和黑眼期(C期)分别检测到81、80和75个蛋白点,特有蛋白质分别为8个、7个和2个,共有蛋白质为61个,A期到B期有4个蛋白质显著上调,5个显著下调,B期到C期有7个蛋白质显著上调,1个显著下调,A期到C期有10个蛋白质显著上调,有4个显著下调。此外,3个蛋白质是在A、B期表达C期关闭,6个蛋白质A、C期表达,B期关闭,5个蛋白质A期关闭,而B、C期表达。初步表明小峰熊蜂蜂王从蛹期发育到成蜂过程中,不仅需要一些保守蛋白质来调控,而且还需要一些特异蛋白质。

Proteome analysis of the pupae development of Bombus hypocrita queen

In order to understand the characteristics of protein expression and regulation in pupae of Bombus hypocrite queens and the molecular mechanism of their development, we investigated the proteome of pupae in different developmental stages using two-dimensional gel electrophoresis. The results show that 81, 80 and 75 proteins were detected in the white-eyed pupal stage （A）, brown-eyed pupal stage （B） and dark-eyed pupal stage （C）, respectively. 8, 7 and 2 proteins were specific to the A, B and C stages, respectively. Meanwhile, 61 proteins were present in all three stages, among which 4 were significantly up-expressed and 5 were significantly down-expressed from the A to B stage, 7 were significantly up-expressed and 1 was significantly down-expressed from the B to C stage, and 10 were significantly up-expressed and 4 significantly down-expressed from the B stage to C stage. In addition, 3 proteins were expressed in both A and B stages but not in C stage, 6 proteins were expressed in A stage, silenced in B stage and expressed in C stage, and 5 proteins were silenced in A stage but were expressed in both B and C stage. Our preliminary conclusion is that the development from pupae to adults of queens of B. hypocrite not only requires the usual regulatory proteins but also the action of special proteins.