绿盲蝽雌虫的浸提物分析

采用浸泡法和气质联(GC-MS)用对绿盲蝽Lygus lucorum Meyer-dür雌虫不同部位的浸提物进行鉴定。结果表明:确定10种成分为绿盲蝽雌虫的浸提物中的主要组分,包括醇类,酸类和酯类。相对含量较高的依次有反-2-丁酸己烯酯、反-2-己烯醇、丁酸、顺-3-己烯醇、丁酸己酯。绿盲蝽雌虫在完成性召唤活动后,丁酸己酯、反-2-丁酸己烯酯和反-2-己烯醇的含量都明显减少,其中反-2-丁酸己烯酯从召唤前的7200ng减少到召唤后的250ng,表明在绿盲蝽雌虫性召唤活动中,有大量的反-2-丁酸己烯酯释放到体外。比较绿盲蝽雌虫的不同部位的浸提物的组分发现反-2-丁酸己烯酯主要存在于虫体胸部,占总量的89.75%,且反-2-己烯醇也主要存在于胸部。因此推测反-2-丁酸己烯酯可能是绿盲蝽雌虫的性信息素成分或组分之一,并主要由胸部内的腺体分泌。

Identification of extracted compounds of female Lygus lucorum

Semiochemicals extracted from virgin females of the green leaf bug,Lygus lucorum Meyer-dür,were analyzed by GC-MS.The volatiles extracted comprised ten compounds identified as esters,alcohols and acids.Among the compounds identified,（E）-2-hexenyl butyrate（E）-2-henenol,butyric acid,hexyl butyrate,and（Z）-3-henenol were the major components.Three compounds,hexyl butyrate,（E）-2-hexenyl butyrate and（E）-2-henenol significantly decreased with increased female calling activity.The fact the amount of（E）-2-hexenyl butyrate decreased from 7200 ng per insect to 250 ng indicated that a large amount of（E）-2-hexenyl butyrate was emitted into the ambient air by females.Comparing the content of（E）-2-hexenyl butyrate in extracts from different parts of females,89.75% was found in the thorax together with（E）-2-henenol.We suggest that（E）-2-hexenyl butyrate is a component of the sex pheromone of female L.lucorum that is secreted by a gland,or glands,in the thorax.