| SRAP技术研究烟粉虱遗传多样性 |
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| 引用本文： | 滕海媛,吴青君,徐宝云,朱国仁,王少丽,张友军.SRAP技术研究烟粉虱遗传多样性[J].昆虫知识,2009,46(1):139-144. |
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| 作者姓名： | 滕海媛 吴青君 徐宝云 朱国仁 王少丽 张友军 |
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| 作者单位： | 中国农业科学院蔬菜花卉研究所,北京,100081 |
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| 基金项目： | 国家重点基础研究发展规划(973计划) |
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| 摘 要： | 采用AFLP、SRAP2种标记方法分别对2个烟粉虱Bemisia tabaci Gennadius种群(一品红、甘蓝)的多态性进行分析。结果表明,(1)2种方法平均每对引物组合产生的条带数分别为29.4和21.8。(2)AFLP法每对引物组合产生10～23条多态性带,平均17.20条,多态性带的比例平均为57.93%。SRAP法每对引物组合产生5～18条多态性带,平均13.3条,多态性带的比例平均为60.59%。(3)前者的基因多样性范围为0.1503～0.2838,平均为0.2297;后者的基因多样性范围为0.0977～0.2911,平均为0.2332。证明利用SRAP技术和AFLP技术研究烟粉虱的遗传多样性是有效的。
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| 关 键 词： | 烟粉虱 多态性 AFLP标记 SRAP标记 |
Polymorphism analysis of Bemisia tabaci by SRAP markers |
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| TENG Hai-Yuan,WU Qing-Jun,XU Bao-Yun,ZHU Guo-Ren,WANG Shao-Li,ZHANG You-Jun.Polymorphism analysis of Bemisia tabaci by SRAP markers[J].Entomological Knowledge,2009,46(1):139-144. |
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| Authors: | TENG Hai-Yuan WU Qing-Jun XU Bao-Yun ZHU Guo-Ren WANG Shao-Li ZHANG You-Jun |
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| Institution: | (Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China) |
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| Abstract: | Polymorphism between poinsettia and cabbage populations was analyzed using SRAP （sequence-related amplified polymorphism） and AFLP （amplified fragment length polymorphism） markers. The results showed that the average amplified fragments per primer were 29.4 and 21.8 by AFLP and SRAP, respectively. There were 10 - 23 clear polymorphic bands per primer combination, with the average of 17.20. The percentage of polymorphic fragments was 57.93% by AFLP. Correspondingly, for SRAP, they were 5 - 18, 13.3 and 60.59%, respectively. The gene diversity of AFLP and SRAP were 0.150 3 -0.283 8 and 0.097 7 - 0.291 1, with the averages of 0.229 7 and 0.233 2, respectively. It seems that both AFLP and SRAP markers were efficient marker system for polymorphism analysis of Bemisia tabaci. |
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| Keywords: | Bemisia tabaci polymorphism SRAP marker AFLP marker |
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