寄主植物对松墨天牛幼虫体内酶系活性的影响

室内采用在马尾松、黑松、火炬松、湿地松和雪松皮下接入松墨天牛Monochamus alternatus Hope幼虫的方法,探明5种寄主植物对松墨天牛幼虫的存活、钻蛀取食及纤维素酶、酯酶、羧酸酯酶活性的影响。结果表明,5种寄主植物对松墨天牛幼虫存活和钻蛀取食有显著影响,接种于马尾松的天牛幼虫存活率100%;接种于雪松的松墨天牛幼虫存活率仅55.56%。松墨天牛取食不同寄主后,其体内纤维素酶、酯酶和羧酸酯酶活性存在显著差异。其中以取食火炬松的Cx酶活性最高,为(4.5754±0.1975)μmol·g-1(FW)·min-1,C1酶和β-l,4-葡萄糖苷酶活性以取食马尾松的松墨天牛幼虫消化道中最高,分别为(2.8199±0.0510)μmol·g-1(FW)·min-1与(3.0793±0.1733)μmol·g-1(FW)·min-1;而以取食黑松和马尾松的酯酶、羧酸酯酶活性最高,分别为1372.74mmoD/min·μg和744.82mmoD/min·μg。

Effect of different host plants on activitities of several enzymes of the larvae of Monochamus alternatus

The effects of host plants on survival,boring behavior,activity of cellulase,esterase and carboxylesterase of larvae of Monochamus alternatus Hope were investigated after the larvae were inoculated under the bark of five plants,Pinus massoniana,P.thunbergi,P.taeda,P.elliottii and Cedrus deodara,respectively.The results showed that host plants significantly affected survival and boring behavior of larvae of M.alternatus.The survival of the larvae fed with P.massoniana was 100%,whereas it was 55.56% which fed with C.deodara.Activities of the cellulase,esterase and carboxylesterase of the larvae were significantly different among the five treatments.The activity of Cx of M.alternatus fed on P.teada was the highest,which was(4.575 4±0.197 5)μmol ·g-1(FW)·min-1,the activity of C_1 and β-l,4-glucosidase of larvae fed on P.massoniana were the highest,which were(2.819 9±0.051 0)μmol ·g-1(FW)·min-1 and(3.079 3±0.173 3)μmol ·g-1(FW)·min-1,respectively.The activities of esterase and carboxylesterase of the larvae fed on P.thunbergii and P.massoniana were the highest,which were 1 372.74 mmoD/min·μg and 744.82 mmoD/min·μg,respectively.