| PCR-SSCP技术在昆虫学研究中的应用 |
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| 引用本文： | 杜波,迟德富.PCR-SSCP技术在昆虫学研究中的应用[J].昆虫知识,2007,44(3):333-336. |
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| 作者姓名： | 杜波 迟德富 |
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| 作者单位： | 东北林业大学林学院,哈尔滨,150040 |
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| 基金项目： | 黑龙江省森林重大病虫害高效安全持续控制技术项目 |
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| 摘 要： | 聚合酶链式反应-单链构象多态性(PCR-SSCP)技术是一种简便、灵敏的突变检测方法。随着该技术的不断发展和完善,其应用也越来越广泛。尤其是近年来该技术在昆虫学研究中涉及了:基因突变位点的检测、昆虫分类鉴定、多样性分析及连锁图谱的构建等多个领域。文章综述PCR-SSCP技术的发现、原理、在昆虫学研究中的应用及其优点和不足之处。
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| 关 键 词： | PCR-SSCP 突变检测 连锁图谱 |
| 修稿时间： | 2006-10-11 |
The application of PCR-SSCP in the research of entomology |
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| DU Bo,CHI De-Fu.The application of PCR-SSCP in the research of entomology[J].Entomological Knowledge,2007,44(3):333-336. |
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| Authors: | DU Bo CHI De-Fu |
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| Institution: | College of Forestry, Northeast Forestry University, Haerbin 150040, China |
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| Abstract: | PCR-SSCP is a simple and sensitive method to screen mutations.This method is applied more and more widely along with its development and maturation. In recent years, PCR-SSCP is used in many fields of Entomology:screening gene mutation, identifying insects, studying the diversity of the genes, and constructing the genetic linkage map.This review includes the principle, new discovery, advantages and disadvantages of PCR-SSCP and finally the applications of the method in entomology is intrduced. |
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| Keywords: | PCR-SSCP mutation detection genetic linkage map |
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