| 芫菁科昆虫体内斑蝥素的气相色谱法测定 |
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| 引用本文： | 李晓飞,陈祥盛,国兴明.芫菁科昆虫体内斑蝥素的气相色谱法测定[J].昆虫知识,2007,44(3):459-462. |
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| 作者姓名： | 李晓飞 陈祥盛 国兴明 |
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| 作者单位： | 1. 贵州大学昆虫研究所,贵阳,550025
2. 贵州大学生化营养研究所,贵阳,550025 |
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| 基金项目： | 贵州省优秀科技教育人才省长资金(15001B-10)，贵州省高层次人才科研条件特助经费，贵阳市大学生创业科技项目资助(2006筑科技合同字第21-2号) |
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| 摘 要： | 分别采用酸水解法和直接浸提法处理不同种芫菁样品、短翅豆芫菁Epicauta aptera Kaszab的卵和大斑芫菁Mylabris phalerata Pallas的不同虫体部位,后用气相色谱仪测定斑蝥素含量。结果表明:用酸水解法处理后的芫菁体内斑蝥素含量较之用直接浸提法处理后有显著提高,增高幅度在1~9倍之间,其中以豆芫菁属Epicauta昆虫的增高幅度最大,一般在7倍以上,而斑芫菁属Mylabris的斑蝥素含量增幅不高,芫菁卵中斑蝥素含量变化不显著;斑蝥素主要富集于大斑芫菁的腹部。
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| 关 键 词： | 斑蝥素 芫菁科 气相色谱法 酸水解法 直接浸提法 |
| 修稿时间： | 2006-02-22 |
Gas-chromatographical study of cantharidin in Meloidae |
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| LI Xiao-Fei,CHEN Xiang-Sheng,GUO Xing-Ming.Gas-chromatographical study of cantharidin in Meloidae[J].Entomological Knowledge,2007,44(3):459-462. |
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| Authors: | LI Xiao-Fei CHEN Xiang-Sheng GUO Xing-Ming |
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| Institution: | 1.Institute of Entomology , Guizhou University, Guiyang 550025, China; 2. Institute of Biochemistry and Nutrition, Guizhou University, Guiyang 550025, China |
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| Abstract: | The samples from different species of Meloidae, the eggs of Epicauta aptera Kaszab and the different parts of Mylabris phalerata Pallas were treated with acidolysis or direct extraction, and cantharidin contents were determined by gas chromatography. The result showed that cantharidn contents in the samples of Meloidae treated with acidolysis method were 1 to 9 times higher than those dealt with extraction method, usually more than 7 times higher in Epicauta, and no much differences in Mylabris. The differences of cantharidin contents in eggs between samples with two methods were not notable. The cantharidin was mainly found in the abdomen of M. phalerata. |
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| Keywords: | cantharidin Meloidae gas chromatography acidolysis direct extraction |
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