| 二种大实蝇的PCR-RFLP快速鉴定方法 |
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| 引用本文： | 林丽莉,吴佳教,曾玲,梁广文,胡学难,莫仁浩.二种大实蝇的PCR-RFLP快速鉴定方法[J].昆虫知识,2007,44(4):588-592. |
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| 作者姓名： | 林丽莉 吴佳教 曾玲 梁广文 胡学难 莫仁浩 |
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| 作者单位： | 1. 华南农业大学昆虫生态研究室,广州,510642
2. 广东检验检疫技术中心,广州,510623 |
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| 基金项目： | 广东省自然科学基金项目资助(04020713)，广东省科技厅科学事业项目资助(2004B20501006) |
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| 摘 要： | 应用PCR-RFLP技术,对我国部分地区发生分布的蜜柑大实蝇和橘大实蝇开展了分子生物学鉴定方法研究。结果表明,利用4组引物对目标实蝇的COⅡ和ITS1基因进行PCR扩增,并借助MnlⅠ,MseⅠ,AseⅠ,DraⅠ和SspⅠ等5种限制性内切酶对扩增产物进行酶切,能从多个途径把2种大实蝇区分开。所建立的方法不受目标实蝇食物源、地理来源和标本保存条件的影响,对不同虫态和不同性别的个体均适用,可在生产和检疫实践中对蜜柑大实蝇和橘大实蝇进行快速鉴定。
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| 关 键 词： | 蜜柑大实蝇 橘大实蝇 PCR-RFLP COⅡ ITS1 |
| 修稿时间： | 2006-08-16 |
Rapid identification of two species of Tetradacus by PCR-RFLP |
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| LIN Li-Li,WU Jia-Jiao,ZENG Ling,LIANG Guang-Wen,HU Xue-Nan,MO Ren-Hao.Rapid identification of two species of Tetradacus by PCR-RFLP[J].Entomological Knowledge,2007,44(4):588-592. |
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| Authors: | LIN Li-Li WU Jia-Jiao ZENG Ling LIANG Guang-Wen HU Xue-Nan MO Ren-Hao |
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| Institution: | 1. Laboratory of Insect Ecology, South China Agricultural University, Guangzhou 510642, China; 2. Guangdong Inspection and Quarantine Technology Center, Guangzhou 510623, China |
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| Abstract: | The rapid molecular diagnostic technique for identification of Bactrocera(Tetradacus) tsuneonis and Bactrocera(Tetradacus) minax was based on PCR-RFLP of the mitochondrial DNA(mtDNA) and ribosomal DNA(rDNA).With four pairs of primers and five restriction endonucleases(MnlⅠ,MseⅠ,AseⅠ,DraⅠ and SspⅠ),these two species can be discriminated according to the restrictive patterns.The result was not affected by the geographical origins,host plants of the fruit flies,or even the life stages,sexes and preservation of the individuals.This method should be useful in production and quarantine application. |
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| Keywords: | PCR-RFLP ITS1 |
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