内蒙古地区亚洲小车蝗不同地理种群的RAPD分析

采用随机扩增多态性DNA(RAPD)技术对内蒙古地区亚洲小车蝗Oedaleus asiaticus(B.-bienko)9个不同地理种群90个个体进行扩增,8条随机引物扩增共产生了78条带,多态性片段为62条。对Nei′s基因多样性指数和遗传距离进行分析,结果表明:种群间的遗传分化系数为0.2343,即23.43%的遗传变异存在于种群间,种群内的遗传分化系数为0.7657,即76.57%的遗传多样性存在于种群内,群体内遗传多样性大于群体间遗传多样性。用NJ法对这3个种群的Nei′s遗传距离作聚类分析,结果表明亚洲小车蝗不同种群的遗传分化程度与地理距离具有正相关关系。

The genetic divergence of nine populations of Oedaleus asiaticus in Mongolia based on RAPD

The genetic divergence of 9 natural populations of Oedaleus asiaticus（B.-bienko） was studied using the RAPD method.Genomic DNA of 90 individuals was amplified with eight random primers.A total of 78 clear and reproducible bands were generated,62 of which were polymorphic,and Nei＇s index and a genetic distance matrix calculated.The gene differentiation coefficient between populations estimated by Nei＇ s index was 0.2343,indicating that 23.43% of the variation occurred between populations.The gene differentiation coefficient within populations was 0.7657,indicating that 76.57% of all variation was within populations.The gene differentiation coefficient within populations was evidently higher than that between populations.A dendrogram based on Nei＇s genetic distance of RAPD markers was constructed using the NJ method.The results indicate that genetic divergence in populations of O.asiaticus corresponds to both geographic and genetic distance.