不同地理飞蝗种群的遗传多样性

用GenBank中飞蝗Locusta migratoriaL.的序列来设计微卫星引物,并对这些引物的有效性进行验证。结果表明,在所设计的3对引物中,只有1对为有效引物,可扩增出微卫星位点。序列分析表明本位点是一个不连续的重复微卫星位点。该多态微卫星位点含有14个等位基因,不同飞蝗地理种群的等位基因数目、大小和频率都存在较大的差异。对该位点各等位基因型进行χ2检验,基因型频率的观察和期望杂合度分别为0.4578和0.8836,该位点不属于Hardy-Weinberg平衡位点(χ2=733.12,P=0.0000)。该微卫星位点表现出高度的多态性说明是分析飞蝗种群遗传多样性的优良分子遗传标记。

Genetic diversity among geographical populations of the migratory locust

In this study,microsatellite primers were designed based on the sequences of migratory locust in Gen Bank and tested for their workability.The results showed that only one of three pairs of primer was workable and could be used to amply one microsatellite locus.The sequence of this locus contained repeat units of(TG)_n and(GC)_n,meaning a discontinuous microsatellite.At this locus there were 14 alleles.The allele number,size and frequency for different locust populations were much different.Chi-squared tests for the genotype frequencies at this locus were performed.The average expected and observed heterozygosity for this locus was 0.883 6 and 0.457 8,respectively,indicating the genotype frequency of this loci deviated from Hardy-Weinberge equilibrium(χ2=733.12,P=0.0000).The highly polymorphism of this microsatellite locus made it suitable to sever as a good molecular genetic marker to analyze genetic difference of geographical populations of migratory locust.