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| 30K蛋白对家蚕细胞及幼虫存活的作用 | |
| 引用本文： | 唐平,李兵,沈卫德,陈劲频,王文兵.30K蛋白对家蚕细胞及幼虫存活的作用[J].昆虫知识,2007,44(6):826-829. |
| 作者姓名： | 唐平 李兵 沈卫德 陈劲频 王文兵 |
| 作者单位： | 1. 江苏大学生命科学研究院,镇江,212013;江苏科技大学生物与环境工程学院,镇江,212018 2. 苏州大学生命科学研究院,苏州,215123 3. 江苏大学生命科学研究院,镇江,212013 |
| 基金项目： | 973课题:家蚕生物工程及基因组应用的拓展研究(2005CB121005)，江苏省教育厅高校科学研究项目:家蚕细胞凋亡的关键基因克隆和鉴定(02KJD18003)，江苏大学自然科学创新预研基金项目(04CX08) |
| 摘 要： | 利用杆状病毒表达系统在家蚕BombyxmoriL.细胞BmN中表达家蚕30K蛋白,以亲本病毒BmPAK6为对照,将重组病毒Bm/r-30K分别感染BmN细胞及家蚕幼虫,观察其感染后不同时间的凋亡及存活率。与对照相比,重组病毒感染后的BmN细胞的存活率明显高于对照组,并且感染的家蚕幼虫存活时间也较长,表明体内过量表达家蚕30K蛋白有助于延长其细胞及幼虫的存活。 |
| 关 键 词： | 家蚕 30K蛋白 杆状病毒 表达 生长 |
| 收稿时间： | 2007-05-17 |
| 修稿时间： | 2007-07-23 |
Influence of 30K protein in Bombyx mori on survival BmN cells and larvae |
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| TANG Ping,LI Bing,SHEN Wei-De,CHEN Jing-Ping,WANG Wen-Bing.Influence of 30K protein in Bombyx mori on survival BmN cells and larvae[J].Entomological Knowledge,2007,44(6):826-829. | |
| Authors: | TANG Ping LI Bing SHEN Wei-De CHEN Jing-Ping WANG Wen-Bing |
| Abstract: | The 30K protein was successfully expressed in the Bombyx mori L.(Bm)cells by baculovirus system.B.mori cells and larvae were infected with the recombinant baculovirus(Bm/r-30K),respectively.The viability of cells and larvae were assayed at various times post-infection and BmPAK6 was used as a control.The viability of BmN cells infected with baculovirus(Bm/r-30K)was higher than that of the cells infected with BmPAK6 virus,the viability and surviving time of B.mori larvae were increased as well.The results indicate that over-expression of the B.mori 30K gene contributes to increase the surviving time of BmN cells and larvae. |
| Keywords: | Bombyx mori 30K protein baculovirus expression longevity |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |