分子生物学技术在双翅目实蝇科昆虫系统发育研究中的应用

分子生物学技术如同工酶电泳、RFLP、RAPD、核酸序列分析、微卫星DNA和探针杂交等,在实蝇科昆虫系统发育研究中具有重要作用。利用这些技术对实蝇种群进行系统发育研究,揭示其亲缘及进化关系,从生命本质上寻找实蝇种群间的内在联系。文章综述上述几种分子生物学技术在实蝇科昆虫核酸结构、种内和种间的亲缘及进化关系等方面的研究进展,分析在应用中存在的问题,展望这些分子生物学技术在实蝇科昆虫系统发育中的应用前景。

Application of molecular biological techniques in insect phylogeny of the family Tephritidae

Molecular biological techniques,such as isozymes electrophoresis,RFLP,RAPD,nucleic acid sequence analysis,micro-satellite DNA and probe hybridization,were applied in the studies of phylogeny in fruit flies since its commencement in 1980's.Evolution relationship and history of fruit flies were revealed in essence by these techniques.This review summarized these molecular biological techniques being used in the studies of insect phylogeny in fruit flies,including genome structure and evolution relationship among intra-species and interspecies.Some problems and perspective of molecular biological techniques were presented.