Rat lens aldose reductase and polyol production. |
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| Authors: | M J Crabbe and S P Wolff |
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| Institution: | Department of Physilogy, Semmelweis University Medical School, Budapest, Hungary. |
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| Abstract: | Bovine anterior-pituitary microsomal fractions exhibit high-affinity, saturable and reversible binding of inositol 1,4,5-32P]trisphosphate; 50% of the labelled ligand is displaced by 3.5 nM-inositol 1,4,5-trisphosphate. 0.5 microM-inositol 1,4-bisphosphate and 10 microM-ATP. Inositol 1,4,5-trisphosphate induces the release of Ca2+ from the microsomal vesicles (half-maximal effect at 290 nM), and its action is potentiated by inositol tetrakisphosphate (half-maximal effect at 4 microM). |
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