Phospholipid synthesis and degradation during the life-cycle of P815Y mast cells synchronized with excess of thymidine

1. P815Y cells synchronized with excess of thymidine incorporate choline, proline and uridine throughout the cell cycle; the rate increases two- to four-fold during the S phase, when thymidine incorporation increases more than 15-fold. 2. Choline incorporated at any stage of the cell cycle turns over in a biphasic manner; stable and unstable components are each labelled maximally during the S phase. Total phospholipid also doubles predominantly during the S phase. 3. It is concluded that, despite turnover, choline incorporation is a useful measure of net phospholipid formation during the cell cycle.