The Ultrastructure of the Fungus Trichoderma viride and Investigation of its Growth on Cellulose |
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Authors: | BJÖRN BERG ANGELICA v HOFSTEN |
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Institution: | Department of Microbiology, Swedish University of Agriculture, Forestry and Veterinary Medicine, Uppsala 7, Sweden;Institute of Physiological Botany, University of Uppsala, S-751 21 Uppsala, Sweden |
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Abstract: | Changes in the ultrastructure of Trichoderma viride during growth in shake cultures on cellobiose and cellulose fibres were examined. Electron micrographs of thin sections of germinating conidia, septate hyphae with ascomycete pores and other cell organelles are presented. Extensive autolysis of hyphae was observed after growth for 20 h on cellobiose. The fungus grew in the lumina and within the walls of cellulose fibres. The hyphae followed the directions of the laminar structure but did not grow across them. The observations indicated that the hyphae penetrated the fibres by causing cracks and by dissolving enzymatically the cellulose. |
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