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中国腹刺斑螟生物学和线粒体COI基因序列初步记述
引用本文:肖云丽,方程,任应党,杨琳琳,钟玉林.中国腹刺斑螟生物学和线粒体COI基因序列初步记述[J].环境昆虫学报,2016(6):1178-1184.
作者姓名:肖云丽  方程  任应党  杨琳琳  钟玉林
作者单位:1. 经济林木种质改良与资源综合利用湖北省重点实验室,大别山特色资源开发湖北省协同创新中心,黄冈师范学院生命科学学院,湖北黄冈438000;2. 河南农业科学院植物保护研究所,郑州,450002
基金项目:湖北省教育厅科学研究项目(D20152904),湖北省自然科学基金项目(2015CFA158),湖北省重点实验室项目(2013000703),湖北省协同创新中心创新团队项目(2015TD06)
摘    要:首次发现中国腹刺斑螟幼虫取食板栗叶片,描述了幼虫和蛹的形态特征,拍摄了幼虫和蛹的生活特征图和外形等结构特征图,初步描记了其在湖北省罗田县栗园的生活习性;对幼虫线粒体COI基因片段序列进行了提取、扩增、测序和分析,其5’端629 bp的DNA序列可以作为中国腹刺斑螟鉴定的分子标记。

关 键 词:中国腹刺斑螟  幼虫    生物学  COI  gene  板栗  中国

Preliminarily notes on biology and mitochondrial COI gene of Sacculocornutia sinicolella Caradja (Lepidoptera: Pyralidae: Phycitinae)
XIAO Yun-Li,FANG Cheng,REN Ying-Dang,YANG Lin-Lin,ZHONG Yu-Lin.Preliminarily notes on biology and mitochondrial COI gene of Sacculocornutia sinicolella Caradja (Lepidoptera: Pyralidae: Phycitinae)[J].Journal of Environmental Entomology,2016(6):1178-1184.
Authors:XIAO Yun-Li  FANG Cheng  REN Ying-Dang  YANG Lin-Lin  ZHONG Yu-Lin
Abstract:Sacculocornutia sinicolella Caradja was firstly reported feeding on the leaves of Castanea mollissima Blume in Luotian County, Hubei Province, China. On the basis of both field survey and laboratory rearing, the larva and pupa morphologies of Sacculocornutia sinicolella were described and photographed, the ecological habits of the species were primarily given and photographed, and molecular analyses based on the 629 bp portion of the mitochondrial DNA from the cytochrome oxidase I ( mt COI) region was conducted to supplement morphological characteristics in identifying this species.
Keywords:Sacculocornutia sinicolella  larva  pupa  biology  COI gene  Castanea mollissima Blume  China
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