| 一种对甲螨无形态损伤的DNA提取技术 |
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| 引用本文： | 陈燕南,范成,张锋,朱朝东,陈军.一种对甲螨无形态损伤的DNA提取技术[J].环境昆虫学报,2022(3):751-755. |
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| 作者姓名： | 陈燕南 范成 张锋 朱朝东 陈军 |
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| 作者单位： | 1. 中国科学院动物研究所动物进化与系统学院重点实验室，北京 100101；2. 河北大学生命科学学院动物系统学与应用重点实验室，河北保定 071002；3. 中国科学院大学，北京 100049 |
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| 基金项目： | 国家科技基础资源调查专项（2019FY101800）；国家自然科学基金（32070421）；“一带一路”国际科学组织联盟联合研究合作专项（ANSO-CR-KP-2020-04） |
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| 摘 要： | 甲螨是一类重要的土壤动物，体型微小，一般具有较厚的体壁。本研究针对甲螨这一特定类群，探讨了一种无形态特征损伤的DNA提取技术。通过结合试剂盒DNA提取法，并适当改进实验条件，设计出一套行之有效的DNA提取流程。通过对提取DNA之后的标本进行形态学观察，发现其主要的分类学特征均保存完好，可以作为凭证标本长期保存。本研究所提供的DNA提取技术既可以提取出足够的DNA又可以保留凭证标本，因此能有效促进甲螨分子分类学相关研究。
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| 关 键 词： | 甲螨 无损 DNA提取 凭证标本 形态特征 |
A new method for DNA extraction without destroying morphological characters applied to oribatid mites |
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| CHEN Yan-Nan,FAN Cheng,ZHANG Feng,ZHU Chao-Dong,CHEN Jun.A new method for DNA extraction without destroying morphological characters applied to oribatid mites[J].Journal of Environmental Entomology,2022(3):751-755. |
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| Authors: | CHEN Yan-Nan FAN Cheng ZHANG Feng ZHU Chao-Dong CHEN Jun |
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| Institution: | 1. Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; 2. Key Laboratory of Zoological Systematics and Application, College of Life Science, Hebei University, Baoding 071002, Hebei Province, China; 3. University of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | Oribatid mites are of great significance in soil ecological system. They are small (0.1~1 mm) and usually sclerotized heavily. This research provided an efficient and non-destructive DNA extraction method for oribatid mites. By changing the method of DNA extraction and using DNeasy blood and tissue kit, a new method for DNA extraction without destroying morphological features could be applied to oribatid mites. The morphological characters valuable for identifying the specimens had not been destroyed after DNA extraction with this method, and the voucher specimen could be kept for further studies. This method could not only obtain high quality DNA templates but also keep voucher specimen. It could be an efficient way to help DNA taxonomy on oribatid mites. |
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| Keywords: | Oribatid mites non-destructive DNA extraction voucher specimen morphological character |
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