高压静电场胁迫对各代麦长管蚜体重及抗氧化酶基因表达的影响

为探究高压静电场对麦长管蚜的影响,设置不同剂量(4 kv/cm-20 min、5 kv/cm-40 min)的高压静电连续处理小麦种子和出生12 h内的麦长管蚜若虫40代,以未经上述处理的小麦种子和蚜虫为对照;通过qRT-PCR的方法对处理至第4,11,21,31,40代的麦长管蚜进行抗氧化酶基因表达量的测定,并测定相近世代麦长管蚜的相对日均体重增长率.结果表明:CuZnSOD、MnSOD、POD 3种抗氧化酶基因均在第4代和第40代上调,第11～31代基因表达不显著或呈下调模式;且第4代麦长管蚜在5 kv/cm-40 min剂量下,上述3种酶基因上调最显著,而在第40代麦长管蚜在4 kv/cm-20 min剂量下,酶基因上调最显著.相对日均体重增长率随世代变化趋势呈先升高后降低趋势,产生变化的关键世代与酶基因一致.长期胁迫下,随世代延长麦长管蚜抗氧化酶基因表达存在差异,且与体重变化差异相符,这为麦长管蚜随世代增加对高压静电场胁迫调整适应和防御规律的研究提供参考.

Effect of high voltage electrostatic field (HVEF)stress on weight and expression of antioxidant enzyme gene in Sitobion avenae (Hemiptera: Aphididae)

In order to study the effect of high voltage electrostatic field on Sitobion avenae (Fabricius), the wheat seeds and S. avenae nymphs (born within 24 h)were treated for 40 generations by exposing them to an HVEF at 4 kv/cm-20 min intensity for 20 min and at 5 kv/cm intensity for 40 min, respectively, and without treatment (0 kv/cm)as control. Gene expression levels of S. avenae were measured by qRT-PCR of 4th, 11th, 21st, 31st, 40th generation, and mean relative growth rate (MRGR)of S. avenae was determined in these similar generations. The expression of three antioxidant enzymes ( CuZnSOD, MnSOD, POD )genes were up regulated in the 4th and 40th generation, and was not significant and even down regulated in the 11th and 31st generation. Moreover, the above three enzyme genes were most significantly up regulated at the dose of 5 kv/cm-40 min in the 4th generation of S.avenae, while enzyme gene was most significantly up regulated at the dose of 4 kv/cm-20 min in the 40th generation of S.avenae. The MRGR increased first and then decreased with generation, and the key generation to change was consistent with the enzyme gene. There were differences in the expression of antioxidant enzymes and MRGR of S. avenae with generation increasing when exposed under HVEF, and it was proved that S. avenae had a certain adjustment and re -defense to long term HVEF stress for maintain the survival evolution.