红火蚁三龄、四龄幼虫肠道菌菌群多样性分析

昆虫体内细菌的多样性对昆虫的消化、营养的吸收、生长发育繁殖具有至关重要的作用.本研究利用16S rRNA基因序列,构建内生菌16S rRNA克隆文库,探明红火蚁3龄幼虫和4龄幼虫种群体内微生物菌群结构并加以对比,采用Miseq高通量测序的方法揭示红火蚁3龄幼虫与4龄幼虫肠道菌的相似性和差异性.结果表明:红火蚁高龄幼虫的两个不同发育阶段肠道菌菌群的结构及组成存在一定的差异性.3龄幼虫肠道菌的物种丰富度和物种多样性都低于4龄幼虫.红火蚁3龄、4龄幼虫体内共有183个OTU,归属于15个门、24个纲、56个目、101个科、151个属,其中3龄幼虫肠道微生物的OTU数目有51个,其优势菌群为支原菌属,比例达到95.11％;4龄幼虫肠道微生物的OTU数目有170个,其优势菌群为变形菌门的7个属,比例达到93.78％,其中优势菌属为肠杆菌属,比例达到37.10％.本研究分析了红火蚁高龄幼虫肠道菌的细菌多样性及3龄幼虫与4龄幼虫两个阶段肠道菌群的群落结构组成差异,为进一步明确红火蚁高龄幼虫肠道菌生理功能和生态学意义具有重要的参考价值.

Diversity analysis of intestinal flora in the third and fourth-instar larvae of Solenopsis invicta

The diverse bacteria in insects play important roles in digestion, nutrition absorption, growth and reproduction in insects. In this study, 16S rRNA gene sequences were used to construct a 16S rRNA clone library of endophytic bacteria. The microbial community structures of the third and fourth-instar larvae of Solenopsis invicta were determined and compared. The similarities and differences between the microbial communities of the third and fourth instar larvae of S. invicta were revealed by high-throughput MiSeq sequencing. The results showed that there were some differences in the structure and composition of intestinal bacteria in the two different stages of development of S. invicta senior larvae. The species abundance and species diversity of the third instar larvae samples were lower than the fourth instar larvae. A total of 183 OTUs were obtained from the third and fourth-instar larvae of S. invicta belonging to 15 phyla, 24 classes, 56 orders, 101 families and 151 genera. Among them, 51 OTUs were obtained from the third-instar larvae, and 170 OTUs were obtained from the fourth-instar larvae. The dominant microbial taxon of the third-instar larvae was the genus Mycoplasma, which accounts for 95.11%. The dominant microbial taxon of the fourth-instar larvae of S. invicta was 7 genera of the Proteobacteria,with a proportion of 93.78%, of which the dominant bacteria was Enterobacter , with a proportion of 37.10%. This study analyzed the bacterial diversity of the intestinal bacteria in the advanced instar larvae of S.invicta and the difference in the composition of the intestinal flora of the third and fourth-instar larvae in order to further clarify the physiological function and ecology of the intestinal bacteria The academic significance had important reference value.