棉田绿盲蝽微生物多样性研究

昆虫在长期的进化过程中与其体内微生物形成了互利共生的关系，共生微生物参与调节寄主的多种生命活动，例如生殖、代谢等。在黄河流域棉区绿盲蝽Apolygus lucorum是棉花的主要害虫之一。为明确绿盲蝽体内共生菌的种类与群落结构，通过HiSeq平台对棉田绿盲蝽体内共生菌的16S rRNA基因V3～V4区进行高通量测序，分析绿盲蝽体内共生菌的种类与多样性。结果显示，变形菌门Proteobacteria(89.18%)、放线菌门Actinobacteria(2.99%)、厚壁菌门Firmicutes(2.48%)为绿盲蝽的3个优势菌门。在属水平上立克次氏体Rickettsia(32.86%)、欧文氏菌属Erwinia(20.21%)为优势菌群。本文初步明确了绿盲蝽体内微生物群落的组成和动态变化，为进一步研究绿盲蝽与共生菌的相互作用提供了基础，为今后从共生菌出发防治绿盲蝽提供新思路。

Microbial diversity of Apolygus lucorum in cotton field

Insects have formed a mutually-beneficial symbiotic relationship with their microorganisms in the long evolutionary process, and the symbiotic microorganisms participate in the various vital movements of the host, such as reproduction, metabolism, etc. Apolygus lucorum is one of the main pests of cotton in the cotton area of north Shandong. In order to identify the species and community structure of symbiotic bacteria in A.lucorum, high-throughput sequencing of V3 ~ V4 regions of 16S rRNA genes of A.lucorum in cotton field was conducted by Hiseq platform, and the species and diversity of symbiotic bacteria in A.lucorum were analyzed. The results showed that Proteobacteria(89.18%), Actinobacteria(2.99%) and Firmicutes(2.48%) were the three most dominant phyla of A. lucorum. At the genus level, Rickettsia(32. 86%) and Erwinia(20.21%) were the most dominant bacteria. In this study, the composition and dynamic changes of microbial community in A.lucorum were preliminarily described, which provided a basis for further study on the interaction between A.lucorum and symbiotic bacteria, and provided a new idea for the control of A.lucorum with symbiotic bacteria.