利用数码相机和Photoshop软件非破坏性测定叶面积的简便方法

采用数码相机获取叶片的数字图像，用Photoshop图像处理软件计算叶面积，并与目前常用的剪纸法和叶面积仪测定法进行比较分析。结果表明，本方法和上述传统测定方法测定结果存在极显著线性相关；不同拍摄分辨率、单位叶面积存贮像素个数和拍摄角度对测定结果无显著影响。和其它方法相比，本方法具有准确、快速、成本低廉、适合非破坏性动态连续观测等优点，适用于植物生理生态学研究中叶面积的测定。

A simple non-destructive method to measure leaf area using digital camera and Photoshop software

A method for measuring leaf area was developed using digital camera and Photoshop software.The measured data from digital camera was compared with the values from paper-cut and instrumental scanning methods and etc.The results showed that there was highly significant correlation between the results of digital image processing method and other traditional methods.The resolution,pixel number of leaf picture and camera angle had no significant influence on the results of the method.Relative low variation,accuracy,no-destructive and low price are the main advantages of the method,which is applicable and reliable in plant physiological ecology research.