解磷菌株B25的筛选、鉴定及其解磷能力

从安徽省铜陵市铜官山尾矿库木贼根际分离筛选出多株解磷细菌，经过多次筛选纯化获得一株解磷能力较好的菌株B25.采用透射电镜观察和DNA分子技术，确定此菌株属于芽孢杆菌属.研究了解磷菌株B25在培养168 h内的解磷能力、溶液pH值以及菌株生长量的变化情况，并比较了B25在不同条件下的解磷能力.结果表明：解磷菌株B25的解磷能力与溶液pH值之间存在微弱的相关性，在碳源为葡萄糖、初始pH值为7.0、培养温度为30 ℃时解磷效果较好.

Estimation of stock biomass and mortality parameters of oriental sucking barb(Garra orientalis)in Nujiang River of Sichuan Province, Southwest China

In October 2006-October 2008,a total of 136 specimens of oriental sucking barb(Garra orientalis) with body length 54-167 mm and body weight 3-114.6 g were collected from Nujiang River.The stock mass of the oriental sucking barb in the River was tentatively estimated by using length-based cohort analysis(LCA),and the Beverton-Holt dynamic model was developed to predict the population dynamics of the oriental sucking barb to support fishery management.The total mortality rate(Z) and the natural mortality rate(M) of the population were 0.511 and 0.238,and the fishing mortality rate(F) and current exploitation rate(E) were 0.273 and 0.534 per year,respectively.In 2006-2008,the average stock of the oriental sucking barb was 1.47×104 kg,with 1.08×106 individuals,and the initial stock was 1.87×104 kg,with 1.38×106 individuals.The analysis of related parameters and relative yield per recruit suggested that the oriental sucking barb in Nujiang River was in over-exploitation.Consequent conservative fishery strategy should be carried out to protect the resources of oriental sucking barb in Nujiang River.