毛乌素沙地不同飞播造林年限土壤真菌群落结构特征

为探究毛乌素沙地不同飞播造林年限对土壤真菌群落结构的影响,以0、6、16、26和36年共5个飞播造林年限的土壤为对象,采用高通量测序技术检测土壤真菌,分析了其群落结构及多样性,并探讨了土壤因子的影响。结果表明:随着飞播年限的增加土壤真菌群落结构发生变化,除Simpson指数无明显变化外,真菌Alpha多样性指数、菌门和菌属种类以及特有的OTU数量均呈现先降低后增加的趋势;PCoA分析显示,不同年限的土壤真菌群落组成具有差异性,且飞播后期差异增大;Pearson相关性分析结果表明,影响土壤真菌Alpha多样性的主要土壤因子有硝态氮和有效磷。飞播造林有利于毛乌素沙地土壤真菌群落结构的改变和多样性的增加,也有利于优势菌群出现,研究结果对毛乌素沙地飞播造林植被恢复效果提供了一定的理论支持。

Soil fungi community structure in Mu Us Sandy Land with different afforestation durations by aerial seeding

We used high-throughput sequencing technology to assess the effects of multi-year aerial seeding(0,6,16,26,and 36 years) on soil fungi community structure and diversity in the Mu Us Sandy Land,and examined the effects of soil factors on soil fungi community structure.The results showed that soil fungi community structure changed with aerial seeding duration.Except for the Simpson diversity index,fungi alpha diversity index,the number of phylum and genus,and the abundance of unique OTUs showed an initial decrease followed by an increasing trend.Principal coordinates analysis showed variations in soil fungi community composition at different aerial seeding years,with increasing differences along with the extension of aerial seeding duration.Results of Pearson correlation analysis showed that nitrate and available phosphorus concentrations were the main soil factors affecting the distribution of soil fungi.We conclude that aerial seeding afforestation improves soil fungi community structure and diversity by enhancing the emergence of dominant fungi groups in the Mu Us Sandy Land.Overall,our results provide support for the beneficial role of aerial seeding afforestation in vegetation restoration in the Mu Us Sandy Land.