利用蚕豆根尖细胞微核技术检测Cu、As污染的诱变性

应用蚕豆根尖细胞微核技术检测Cu、As及其复合污染的诱变性能,计数了蚕豆根尖细胞微核千分率(MCN‰)和污染指数(PI)并进行了F检验.结果表明,在一定浓度范围内(Cu＜200mg·L,As＜15mg·L^-1),随着Cu、As浓度的增加,MCN‰上升;24个试验处理组PI均在2以上,各处理组MCN‰有显著差异,和对照组有极显著差异(a＜0,01),表明Cu、As及其复合处理对蚕豆根尖细胞的分裂活动有明显影响.

Utilization of micronucleus test in Vicia faba root tips cell to detect the mutability of Cu and As pollution

The micronucleus test in Vicia faba root tips cell was utilized to detect Cu,As and their combined pollution.The MCN‰ and PI were determined and the F test was used to evaluate the statistical difference in MCN‰ among differente treatments.The results showed that within a certain concentration of Cu²⁺ (<200mg· L^-1) and As³⁺ (<15 mg·L^-1), MCN‰ increased as the concentrat io ns increased.The PI of all the 24 treatment was over 2, and there existed obviou s difference compared with the control (α<0.01abstractThis study shows that Cu, As and combined pollution had significant effects on cytogenetical toxicity of Vicia faba root tip cell.MCN‰ is lower than normal level in the complex treatment of Cu and As because of antagonism actions.