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青岛市秋季空气微生物群落多样性
引用本文:章磊,易齐涛,李慧,徐鑫,王晓萌.青岛市秋季空气微生物群落多样性[J].生态学杂志,2015,26(4):1121-1129.
作者姓名:章磊  易齐涛  李慧  徐鑫  王晓萌
作者单位:(青岛理工大学, 山东青岛 266033)
基金项目:国家自然科学基金项目(41202242);淮北股份矿业有限公司2012年度科学技术研究项目资助
摘    要:采用KC-6120空气综合采样器采集空气微生物样品,通过构建16S/18SrDNA克隆文库方法分析青岛市市区街道秋季空气微生物群落结构特征.结果表明: 空气细菌分布在6大类,分别为变形菌门(78.8%)、厚壁菌门(14.6%)、放线菌门(4.0%)、浮霉菌门(1.3%)、蓝藻门(0.7%)和栖热菌门(0.6%),优势菌属为不动杆菌属(39.7%)、葡萄球菌属(11.3%)、鞘脂单胞菌属(8.6%)和副球菌属(6.0%).空气真菌分布在子囊菌门(97.5%)和担子菌门(2.5%),优势菌属为核腔菌属(76.5%)、炭角菌属(13.6%)和外瓶霉属(2.5%).空气微生物中存在不动杆菌属、鞘脂单胞菌、葡萄球菌等致病菌或条件致病菌,以及引发多种农作物枯萎死亡的麦类核腔菌、团炭角菌和角状平脐疣孢等真菌.

关 键 词:克隆文库    空气细菌    空气真菌    群落结构    微生物多样性

Water quality and water environmental capacity for the small subsidence lakes in the Huainan and Huaibei coal mine areas,Anhui Province
ZHANG Lei;YI Qi-tao;LI Hui;XU Xin;WANG Xiao-meng.Water quality and water environmental capacity for the small subsidence lakes in the Huainan and Huaibei coal mine areas,Anhui Province[J].Chinese Journal of Ecology,2015,26(4):1121-1129.
Authors:ZHANG Lei;YI Qi-tao;LI Hui;XU Xin;WANG Xiao-meng
Institution:(Institute of Environmental and Municipal Engineering, Qingdao Technological University, Qingdao 266033, Shandong, China)
Abstract:To determine the community structure of airborne microbes in Qingdao downtown in autumn, the airborne bacteria and fungi were collected by the KC 6120 air sampler and analyzed using the 16S/18S rDNA gene clone library method. Phylogenetic analysis of airborne bacteria showed that they belonged to six major phylogenetic groups: Proteobacteria (78.8%), Firmicutes (14.6%), Actinobacteria (4.0%), Planctomycetes (1.3%), Cyanobacteria (0.7%), and Deinococcus Thermus(0.7%). The dominant genera of airborne bacteria included Acinetobacter (39.7%), Staphylococcus (11.3%), Sphingomonas (8.6%), Paracoccus (6.0%) and Massilia
(5.3%). The main types of airborne fungi were Ascomycota (97.5%) and Basidiomycota (2.5%). Dominant genera of airborne fungi included Pyrenophora (76.5%), Xylaria (13.6%) and Exophiala (2.5%). The pathogens or conditioned pathogens, such as Acinetobacter, Staphylococcus, or Sphingomonas were detected in the airborne bacteria, whereas certain kinds of fungi, such as P. graminea, X. hypoxylon and Zasmidium angulare that could cause a variety of crop diseases were also detected.
Keywords:clone library  airborne bacteria  airborne fungi  community structure  microbial diversity  
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