| 背角无齿蚌基因组(GT)n微卫星DNA特征 |
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| 引用本文： | 汪桂玲,苏翔,李家乐,白志毅.背角无齿蚌基因组(GT)n微卫星DNA特征[J].生态学杂志,2011,30(1):1-6. |
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| 作者姓名： | 汪桂玲 苏翔 李家乐 白志毅 |
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| 作者单位： | 1. 海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室,上海,201306
2. 海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室,上海,201306;上海市高校水产养殖学E-研究院,上海,201306 |
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| 基金项目： | 国家自然科学基金项目(30871923); 上海市科委地方院校能力建设项目(08390510100); 上海市教育发展基金会晨光计划项目(2008CG56)资助 |
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| 摘 要： | 利用磁珠富集法筛选背角无齿蚌(Anodonta woodiana)的微卫星分子标记,采用Sau3A1酶对完整DNA进行酶切,以生物素标记的(GT)15寡核苷酸探针从酶切片段中筛选微卫星序列.洗脱的杂交片段克隆到PGEM-T载体上构建富集微卫星基因组文库后,通过菌液PCR筛选检测出阳性克隆进行测序.结果表明:在筛选的18...
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| 关 键 词： | 背角无齿蚌 磁珠富集法 微卫星 |
Characterization of (GT)n microsatellite in Anodonta woodiana genome |
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| WANG Gui-ling,SU Xiang,LI Jia-le,BAI Zhi-yi.Characterization of (GT)n microsatellite in Anodonta woodiana genome[J].Chinese Journal of Ecology,2011,30(1):1-6. |
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| Authors: | WANG Gui-ling SU Xiang LI Jia-le BAI Zhi-yi |
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| Institution: | WANG Gui-ling1,SU Xiang1,LI Jia-le1,2,BAI Zhi-yi1(1Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Shanghai Ocean University,Ministry of Education,Shanghai 201306,China,2Aquaculture Division,E-Institute of Shanghai Universities,China) |
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| Abstract: | The method of magnetic-bead hybridization enrichment was used to isolate the microsatellite loci of Anodonta woodiana. Genomic DNA was digested with restriction enzyme Sau3A1. The fragments were hybridized with biotin-labeled (GT)15 probe and captured by streptavidin-coated magnetic beads. The target fragments were inserted to PGEM-T vectors,and positive clones were obtained through PCR screening the library. By isolating the (GT) microsatellite in A. woodiana genome,180 positive clones were sequenced,37 se... |
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| Keywords: | Anodonta woodiana magnetic-bead enrichment method microsatellite (SSR) |
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