| 斑节对虾两个野生亲虾种群遗传多样性 |
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| 引用本文： | 谭树华,王桂忠,李少菁.斑节对虾两个野生亲虾种群遗传多样性[J].生态学杂志,2011,30(9). |
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| 作者姓名： | 谭树华 王桂忠 李少菁 |
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| 作者单位： | 1. 湖南科技大学生命科学学院,湖南湘潭411201;厦门大学海洋与环境学院,福建厦门361005
2. 厦门大学海洋与环境学院,福建厦门,361005 |
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| 基金项目： | 国家自然科学基金，湖南省教育厅资助科研项目 |
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| 摘 要： | 采用RAPD和mtDNA 16S rRNA基因序列分析方法对海南和马来西亚斑节对虾(Penaeus monodon)种群进行了遗传多样性和遗传分化研究.结果表明:15个RAPD随机引物共检测到82个位点,海南和马来西亚种群的多态位点比例分别为75.90％和76.83％,杂合度分别为0.199和0.218,遗传多样性指数分别为0.276和0.288,种群间的遗传距离为0.015；16S rRNA基因检测的种内遗传变异较低,马来西亚和海南种群的核苷酸多样性分别为0.011和0,种群之间的遗传距离为0.008；马来西亚种群比海南种群的遗传变异水平要高得多,且海南种群可能起源于马来西亚种群；进行遗传选育时可考虑引进马来西亚亲虾作为奠基群体.
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| 关 键 词： | 斑节对虾 RAPD 16S rRNA 遗传多样性 遗传分化 |
Genetic diversity of two wild Penaeus monodon broodstock populations. |
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| TAN Shu-hua,WANG Gui-zhong,LI Shao-jing.Genetic diversity of two wild Penaeus monodon broodstock populations.[J].Chinese Journal of Ecology,2011,30(9). |
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| Authors: | TAN Shu-hua WANG Gui-zhong LI Shao-jing |
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| Institution: | TAN Shu-hua1,2,WANG Gui-zhong 2,LI Shao-jing2(1 School of Life Sciences,Hunan University of Science and Technology,Xiangtan 411201,Hunan,China,2 College of Oceanography and Environmental Science,Xiamen University,Xiamen 361005,Fujian,China) |
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| Abstract: | By using RAPD and mtDNA 16S rRNA gene sequence analysis,this paper studied the genetic diversity and differentiation of two Penaeus monodon wild broodstock populations collected from Hainan and Malaysia.Using 15 random primers,a total of 82 RAPD loci were detected.For the Hainan and Malaysia populations,the proportion of polymorphic loci was 75.90% and 76.83%,heterozygosity was 0.199 and 0.218,and genetic diversity index was 0.276 and 0.288,respectively,and the genetic distance between the two populations w... |
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| Keywords: | Penaeus monodon RAPD 16S rRNA genetic diversity genetic differentiation |
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