萘降解细菌的分离及其降解基因的分子检测

从污水处理厂的活性污泥和石油工业废水中各分离到24个降解萘的细菌分离株,提取这些分离株的总DNA,然后与各种萘降解基因杂交。结果表明,这2个来源的分离株在萘降解基因的种类上有明显不同。来自工业废水的分离株含有萘双加氧酶的铁硫蛋白大亚基基因nahAc,水杨醛脱氢酶基因nahF及其重复基因nahV,水杨酸羟化酶基因nahG及其重复基因nahU,儿茶酚2,3-双加氧酶基因nahH和儿茶酚1,2-双加氧酶基因catA,以及萘趋化蛋白基因nahY。来自活性污泥的分离株只含有nahAc、nahF、nahG和catA,不含有nahY、nahV、nahU和nahH。

Isolation of naphthalene-degrading bacteria and molecular detection of their naphthalene-catabolic genes

In this paper,24 naphthalene-degrading bacterial isolates were isolated respectively from wastewater treatment facility's activated sludge and from petroleum wastewater.The total DNA of these isolates were extracted,and hybridized with several naphthalene-catabolic genes.The results indicated that the bacterial isolates isolated from the two sources were quite different in their contained naphthalene-degrading genes.The isolates from petroleum wastewater contained naphthalene dioxygenase Fe-S protein large subunit gene nahAc,salicylaldehyde dehydrogenase gene nahF and its duplicate gene nahV,salicylate hydroxylase gene nahG and its duplicate gene nahU,catechol 2,3-dioxygenase gene nahH,catechol 1,2-dioxygenase gene catA,and naphthalene chemotaxis protein gene nahY,while those from activated sludge only contained nahAc,nahF,nahG and catA,but no nahY,nahV,nahU and nahH.