Functional dissection of the Suppressor of UnderReplication protein of Drosophila melanogaster: identification of domains influencing chromosome binding and DNA replication |
| |
| Authors: | T D Kolesnikova I V Makunin E I Volkova V Pirrotta E S Belyaeva I F Zhimulev |
| |
| Institution: | (1) Head of the Laboratory of Molecular Cytogenetics, Institute of Cytology and Genetics SB RAS, Lavrentyev Ave. 10, 630090 Novosibirsk, Russia;(2) University of Geneva, 1211 Geneva, Switzerland;(3) Institute for Molecular Bioscience, University of Queensland, Australia |
| |
| Abstract: | The Suppressor of UnderReplication (SuUR) gene controls the DNA underreplication in intercalary and pericentric heterochromatin of Drosophila melanogaster salivary gland polytene chromosomes. In the present work, we investigate the functional importance of different regions of
the SUUR protein by expressing truncations of the protein in an UAS–GAL4 system. We find that SUUR has at least two separate
chromosome-binding regions that are able to recognize intercalary and pericentric heterochromatin specifically. The C-terminal
part controls DNA underreplication in intercalary heterochromatin and partially in pericentric heterochromatin regions. The
C-terminal half of SUUR suppresses endoreplication when ectopically expressed in the salivary gland. Ectopic expression of
the N-terminal fragments of SUUR depletes endogenous SUUR from polytene chromosomes, causes the SuUR
− phenotype and induces specific swellings in heterochromatin. |
| |
| Keywords: | Drosophila melanogaster GAL4– UAS pericentric and intercalary heterochromatin system suppressor of underreplication protein |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
