Distribution of transposable elements in neotropical species of Drosophila |
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Authors: | EL da S Loreto L Basso da Silva A Zaha VL da S Valente |
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Institution: | (1) Dep. de Biologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil;(2) Dep. de Genética, Caixa Postal 15053, Universidade Federal do Rio Grande do SulCEP, 91501-970, Porto Alegre, RS, Brazil;(3) Dep. de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil |
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Abstract: | The phylogenetic distribution of transposable families, P, gypsy, hobo, I, and mariner has been analyzed in 33 species of
11 groups of neotropical Drosophila and a Drosophilidae species Zygotrica vittimaculosa, using squash blot and dot blot. Genomic
DNA of almost all neotropical species tested hybridized with gypsy probe and some species showed a particularly strong hybridization
signal, as D. gaucha, D. virilis, and species of flavopilosa group. The hobo element was restricted to melanogaster group
and some strains of D. willistoni. Only D. simulans DNA showed hybridization to mariner probe in all species tested and D.
simulans and D. melanogaster showed hybridization with I element probe. P element homologous sequence was present in D. melanogaster
and all species and strains of the willistoni and saltans groups tested. The presence of at least one P-homologous sequence
was detected in Drosophila mediopunctata. This one was the only P-bearing species of all six tested from the tripunctata group.
Four different pairs of primers homologous to segments of the canonical sequence of D. melanogaster's P were used to amplify
specific sequences from D. mediopunctata DNA, showing the occurrence of seemingly well-conserved P-homologous sequences.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | transposable elements Drosophila gypsy hobo P element mariner I element |
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