| 甲型H1N1流感病毒HA基因RT-LAMP检测方法的建立与初步应用 |
| |
| 引用本文： | 王翔,张骞,张钫,袁武梅,麻粉莲,薛鹏浩,赵智慧,郑文芝,郑丽舒.甲型H1N1流感病毒HA基因RT-LAMP检测方法的建立与初步应用[J].生物技术通讯,2011,22(5):696-699,708. |
| |
| 作者姓名： | 王翔 张骞 张钫 袁武梅 麻粉莲 薛鹏浩 赵智慧 郑文芝 郑丽舒 |
| |
| 作者单位： | 中国疾病预防控制中心病毒病预防控制所,病毒基因工程国家重点实验室,北京100052 |
| |
| 基金项目： | 国家传染病防治科技重大专项(2009ZX10004-101,2008ZX10004-002) |
| |
| 摘 要： | 目的:建立反转录环介导等温扩增技术(RT-LAMP)检测甲型H1N1流感病毒HA基因的方法,并用于检测临床样本.方法:通过在线软件Primer Explorer V4设计H1N1 HA基因的RT-LAMP引物,建立RT-LAMP检测方法,并评价其灵敏度和特异度.结果:与传统RT-PCR方法相比,RT-LAMP检测方法具备更高的灵敏度,达到10个拷贝,并且具有良好的特异性；在76份呼吸道感染儿童的咽拭子标本中检测到2份阳性,与RT-PCR方法检测结果相同.结论:建立的H1N1 RT-LAMP检测方法灵敏特异,简单快速,具备H1N1现场检测应用前景.
|
| 关 键 词： | 甲型H1N1流感病毒 RT-PCR RT-LAMP |
Establish and Preliminary Application of RT-LAMP Technique in Detection for Influenza A(H1N1) HA Gene |
| |
| WANG Xiang,ZHANG Qian,ZHA NG Fang,YUAN Wu-Mei,MA Fen-Lian,XUE Peng-Hao,ZHAO Zhi-Hui,ZHENG Wen-Zhi,ZHENG Li-Shu.Establish and Preliminary Application of RT-LAMP Technique in Detection for Influenza A(H1N1) HA Gene[J].Letters in Biotechnology,2011,22(5):696-699,708. |
| |
| Authors: | WANG Xiang ZHANG Qian ZHA NG Fang YUAN Wu-Mei MA Fen-Lian XUE Peng-Hao ZHAO Zhi-Hui ZHENG Wen-Zhi ZHENG Li-Shu |
| |
| Institution: | WANG Xiang,ZHANG Qian,ZHANG Fang,YUAN Wu-Mei,MA Fen-Lian,XUE Peng-Hao,ZHAO Zhi-Hui,ZHENG Wen-Zhi,ZHENG Li-Shu State Key Laboratory for Molecular Virology and Genetic Engineering,National Institute for Viral Disease Control and Prevention,China CDC,Beijing 100052,China |
| |
| Abstract: | Objective: To establish the methods of RT-LAMP assay in influenza virus A(H1N1) HA gene and use to detect clinical samples.Methods: RT-LAMP primer for HA of H1N1 was designed by using Primer Ex-plorer V4 on line,and the sensitivity and specificity of the established assay was evaluated.Results: Comparing with traditional RT-PCR,RT-LAMP showed fine specificity and higher sensitivity until 10 copy.By RT-LAMP as-say we got 2 positive samples in 76 clinical swabs from the children with respiratory infections.Co... |
| |
| Keywords: | influenza virus A(H1N1) RT-PCR RT-LAMP |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
