| 动物活体成像生物发光稳定细胞株的构建 |
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| 引用本文： | 蒋凯,韩永健,程龙,徐小洁,张浩,曹佳,范忠义,叶棋浓.动物活体成像生物发光稳定细胞株的构建[J].生物技术通讯,2011,22(2):216-219. |
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| 作者姓名： | 蒋凯 韩永健 程龙 徐小洁 张浩 曹佳 范忠义 叶棋浓 |
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| 作者单位： | 军事医学科学院,生物工程研究所,北京,100850 |
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| 基金项目： | 国家自然科学基金(81072173); 全军医药卫生科研基金(06J021) |
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| 摘 要： | 目的:构建组成型表达萤光素酶基因的载体,建立稳定高表达萤光素酶的MCF-7乳腺癌细胞株,检测其对细胞增殖的影响及在传代后的表达效果.方法:以载体pGIA.20为模板,PCR扩增萤火虫萤光素酶基因,将其克隆到载体pIRESpuro2上,将获得的pIRESpuro2-Luc经酶切和测序验证后,转染293T、MCF-7及ZR...
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| 关 键 词： | 萤光素酶 稳定细胞株 活体成像 生物发光 乳腺癌 |
Establishment of Stable Cell Lines for Optical In Vivo Imaging in Animal with Bioluminescence |
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| JIANG Kai,HAN Yong-Jian,CHENG Long,XU Xiao-Jie,ZHANG Hao,CAO Jia,FAN Zhong-Yi,YE Qi-Nong.Establishment of Stable Cell Lines for Optical In Vivo Imaging in Animal with Bioluminescence[J].Letters in Biotechnology,2011,22(2):216-219. |
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| Authors: | JIANG Kai HAN Yong-Jian CHENG Long XU Xiao-Jie ZHANG Hao CAO Jia FAN Zhong-Yi YE Qi-Nong |
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| Institution: | JIANG Kai,HAN Yong-Jian,CHENG Long,XU Xiao-Jie,ZHANG Hao,CAO Jia,FAN Zhong-Yi,YE Qi-Nong Beijing Institute of Biotechnology,Beijing 100850,China |
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| Abstract: | Objective:To construct a vector which constitutively expresses firefly luciferase,to establish a stable MCF-7 breast cancer cell line with the luciferase vector,and to detect the influence of the luciferase on the growth of MCF-7 cells after several generations.Mehods:The firefly luciferase gene,amplified with pGL4.20 as a template,was inserted into pIRESpuro2.After confirmation by restriction digestion and DNA sequencing,the vector pIRESpuro2-Luc was transfected into 293T,MCF-7 and ZR75-1 cells to test the... |
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| Keywords: | luciferase stable cell lines optical in vivo imaging bioluminescence breast cancer |
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