| 酶联免疫斑点法在新发传染病疫苗研究中的应用 |
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| 引用本文： | 马玉媛,郭逸,郑霖,田克恭,章金刚.酶联免疫斑点法在新发传染病疫苗研究中的应用[J].生物技术通讯,2011,22(1):135-138. |
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| 作者姓名： | 马玉媛 郭逸 郑霖 田克恭 章金刚 |
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| 作者单位： | 1. 军事医学科学院好战输血研究所/国家生物医学分析中心,病毒安全检测实验室,北京,100850
2. 中国动物疫病预防控制中心,北京,100094 |
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| 基金项目： | 国家科技支撑计划(2007BAD86B01-3) |
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| 摘 要： | 酶联免疫斑点法(ELISPOT)是一项具有较高敏感性和特异性的细胞免疫学检测技术,目前常用于疫苗开发中评价疫苗诱发的细胞免疫效应。我们就ELISPOT技术的原理、具体实验操作、技术优势,及其在新发传染病疫苗研究中的应用等进行综述。
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| 关 键 词： | 酶联免疫斑点法 新发传染病 疫苗 |
Application of Enzyme Linked Immunospot Assay in Emerging Infectious Diseases Vaccines Research |
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| MA Yu-Yuan,GUO Yi,MENG Lin,TIAN Ke-Gong,MANG Jin-Gang.Application of Enzyme Linked Immunospot Assay in Emerging Infectious Diseases Vaccines Research[J].Letters in Biotechnology,2011,22(1):135-138. |
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| Authors: | MA Yu-Yuan GUO Yi MENG Lin TIAN Ke-Gong MANG Jin-Gang |
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| Institution: | MA Yu-Yuan1,GUO Yi1,ZHENG Lin1,TIAN Ke-Gong2,ZHANG Jin-Gang1 1.Laboratory for Viral Safety of National Centre of Biomedical Analysis,Institute of Transfusion Medicine,Academy of Military Medical Sciences,Beijing 100850,2.China Animal Disease Control Center,Beijing 100094,China |
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| Abstract: | Enzyme linked immunospot assay(ELISPOT) is a detection method in cellular immunology with high sensitivity and specificity.This assay is widely used in the development of vaccines for evaluating cell immune responses induced by vaccines.In this review we introduced the principle,procedure,advantages and application in emerging infectious diseases vaccines of ELISPOT. |
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| Keywords: | enzyme linked immunospot assay emerging infectious diseases vaccine |
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