酶联免疫吸附试验定量检测血清肝素酶方法的建立及应用

目的：建立一种简便、微创的血清肝素酶酶联免疫吸附（ELISA）定量检测方法，并对肿瘤患者和正常人血清肝素酶进行比较，初步探讨血清肝素酶水平与肿瘤发生、发展的关系。方法：选择鼠抗人肝素酶单克隆抗体和兔抗人肝素酶多克隆抗体，建立双抗夹心ELISA检测方法，并利用此方法检测健康献血者和肿瘤患者血清中的肝素酶水平。结果：组内数据稳定，可重复；肿瘤患者血清中肝素酶D450nm值高于健康献血者。结论：所建立的血清肝素酶ELISA检测方法灵敏、高效，可用于肿瘤的辅助诊断。

Establishment and Application of ELISA Method for Detecting of Human Serum Heparanase

Objective： To establish a simple, minimally invasive serum heparanase quantitative ELISA detection methods,and preliminary study the relations of the serum heparanase levels and tumor occurrence and development. Methods： The mouse anti-human heparanase monoclonal antibody and rabbit anti-human heparanase polyclonal antibody were used as the first antibodies of an antibody-sandwich ELISA. Heparanase levels of healthy blood donors and cancer patients serum were detected using this ELISA method. Results： D450nm values of tumor patients are significantly higher than those of healthy blood donors. Conclusion： ELISA method for detecting of human serum heparanase is sensitive and high-performance, it can be used for tumor diagnosis.