单域重链抗体的分子特征

源于软骨鱼或骆驼科动物的同型重链二聚体无轻链,用基因工程方法获得其单一重链可变区可保留完整的抗原结合活性,称为单域重链抗体。单域重链抗体具有分子小、稳定性高、体内组织渗透性好、可溶性好、易表达、抗原识别表位独特的结构特征,近年来在生物技术研究与诊断治疗应用领域得到广泛关注,取得了快速发展。

Molecular Characterization of Single Domain Heavy Chain Antibody

Single domain heavy chain antibodies with fully functional antigen-binding fragment are cloned from the single variable domains of homodimeric lacking of light chains within cartilaginous fish and camelids by genet ic engineering approaches.The advantageous structure features of single domain heavy chain antibodies include their small dimension,high apparent stability,good tissue penetration in vivo,improved solubility,high expression,and the singularity of recognizing epitopes.The field of single domain heavy chain antibodies technology has rapid ly progressed during the most recent years,mainly because of the interest in their using in biotechnological re search and diagnostic and therapeutic applications.