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| 腹腔注射百草枯构建小鼠肺纤维化模型 | |
| 引用本文： | 祝春青,陈冬波,王喆,王慧娜,葛翠翠,刘兵,邱泽武,吕双红.腹腔注射百草枯构建小鼠肺纤维化模型[J].生物技术通讯,2012,23(4):563-566. |
| 作者姓名： | 祝春青 陈冬波 王喆 王慧娜 葛翠翠 刘兵 邱泽武 吕双红 |
| 作者单位： | 1. 安徽医科大学解放军307医院临床学院,安徽合肥 230032;军事医学科学院附属医院 消化中毒科,北京 100071;军事医学科学院附属医院 肿瘤学研究室,北京 100071 2. 军事医学科学院附属医院 肿瘤学研究室,北京,100071 3. 军事医学科学院附属医院 消化中毒科,北京,100071 4. 安徽医科大学解放军307医院临床学院,安徽合肥,230032 |
| 基金项目： | 北京市自然科学基金(7112099) |
| 摘 要： | 目的:探讨百草枯一次性腹腔注射致小鼠肺纤维化的病理改变及半数致死剂量(LD50),进而制备肺纤维化病理改变稳定的百草枯中毒小鼠肺纤维化模型。方法:60只正常雌性C57BL/6J小鼠被随机分为6组,10只/组,分别为正常对照组及百草枯给药30、40、50、60和80 mg/kg组,所有小鼠于造模后28 d处死,取其左肺用于病理观察(HE染色),并计算LD50及各组肺纤维化Ashcroft评级。结果:至观察期28 d,小鼠一次性腹腔注射百草枯溶液的LD50为55.1923 mg/kg;各染毒组均出现不同程度的肺纤维化改变,且注射剂量越高,肺纤维化病变越严重,但早期死亡率亦越高。结论:一次性腹腔注射百草枯可制备小鼠肺纤维化模型,40和50 mg/kg为较合适的造模剂量。 |
| 关 键 词： | 百草枯 腹腔注射 肺纤维化 小鼠模型 |
Experimental Study on the Mouse Model of Paraquat Poisoning In duced Pulmonary Fibrosis by Intraperitoneal Injection of Paraquat |
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| ZHU Chu-Qing , CHEN Dong-Bo , WANG Zhe , WANG Hui-Na , GE Cui-Cui , LIU Bing , QIU Ze-Wu , L Shuang-Hong.Experimental Study on the Mouse Model of Paraquat Poisoning In duced Pulmonary Fibrosis by Intraperitoneal Injection of Paraquat[J].Letters in Biotechnology,2012,23(4):563-566. | |
| Authors: | ZHU Chu-Qing CHEN Dong-Bo WANG Zhe WANG Hui-Na GE Cui-Cui LIU Bing QIU Ze-Wu L Shuang-Hong |
| Institution: | ZHU Chu-Qing1,2a,2b,CHEN Dong-Bo2b,WANG Zhe2a,WANG Hui-Na2b,GE Cui-Cui1,LIU Bing2b,QIU Ze-Wu2a*,Lü Shuang-Hong2b* 1.Clinical College,307th Hospital of PLA of Anhui Medical University,Hefei 230032;2.a.Department of Gastro enterology and Poisoning;b.Laboratory of Oncology,Affiliated Hospital of Academy of Military Medical Sciences,Beijing 100071;China |
| Abstract: | Objective: To establish a stable mouse model of paraquat poisoning induced pulmonary fibrosis by a single intraperitoneal injection of paraquat.Methods: 60 healthy female C57BL/6J mice were randomly divided in to six groups according to the paraquat dosage: the saline control group,and the 30,40,50,60 and 80 mg/kg paraquat group,each group of 10 mice.The mice were sacrificed on day 28 for pathological examination(HE stain ing).The LD50 was calculated and the sections after HE staining were evaluated for the formation of lung fibrosis by using Ashcroft scoring system.Results: The LD50 of intraperitoneal paraquat of 28 days was 55.1923 mg/kg.All the paraquat dosage could induce some degrees of pulmonary fibrosis.The severity and early mortality in creased with the dosage.Conclution: We successfully established a mouse model of pulmonary fibrosis by single intraperitoneal injection of paraquat.The optimal paraquat dosage is 40 or 50 mg/kg. |
| Keywords: | paraquat Iintraperitoneal injection pulmonary fibrosis mouse model |
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