| 人肾细胞癌细胞阳离子脂质体的转染效率 |
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| 引用本文： | 高江平,郝好杰,宋涛,赵亚力,谷志远,王晓雄.人肾细胞癌细胞阳离子脂质体的转染效率[J].生物技术通讯,2000,11(1):26-27. |
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| 作者姓名： | 高江平 郝好杰 宋涛 赵亚力 谷志远 王晓雄 |
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| 作者单位： | 1. 解放军总医院泌尿外科,北京,100853
2. 解放军总医院分子生物学教研室 |
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| 摘 要： | 以ＭＴＳ染色法测定实验剂量的Ｌｉｐｏｆｅｃｔｉｎ对细胞的毒性作用，以β－半乳糖苷酶基因为报告基因，通过Ｌｉｐｏｆｅｃｔｉｎ而转染，用Ｘ－ｇａｌ染色法，测定转染效率，结果表明实验剂量（１０μｇ／ｍｌ）的Ｌｉｐｏｆｅｃｔｉｎ对细胞生长无明显毒性。Ｌｉｐｏｆｅｃｔｉｎ对多数肾细胞癌细胞的转染是有效的，且转染效率随Ｌｉｐｏｆｅｃｔｉｎ 度的增高（２．５－１０μｇ／ｍｌ）而增高，说明Ｌｉｐｏｆｅｃｔｉｎ可安
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| 关 键 词： | 肾细胞癌 脂质体 细胞转染 转染效应 基因治疗 |
| 修稿时间： | 1999-12-09 |
Cationic liposome transfection efficiency on human renal cell carcinoma cell line |
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| Gao Jiangping,Hao Haojie,Song Tao,Zhao Yali,Gu Zhiyuan,Wang Xiaoxiong.Cationic liposome transfection efficiency on human renal cell carcinoma cell line[J].Letters in Biotechnology,2000,11(1):26-27. |
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| Authors: | Gao Jiangping Hao Haojie Song Tao Zhao Yali Gu Zhiyuan Wang Xiaoxiong |
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| Abstract: | To evaluate the
efficiency of cationic liposome on human renal cell carcinoma (RCC) cell line. The
cytotoxicity of Lipofectin in vitro was determed by colorimetric method. The transfectin
efficiency was assessed by beta-galactosidase reporter gene expression and X-gal staining.
No significant cytotoxicity of Lipofectin to cultured cell was observed. Lipofectin showed
dose-dependent efficient transfection in wide variety of RCC cell lines.The results show
that safe and effective gene transfer can be achieved using cationic liposome. |
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| Keywords: | renal cell carcinoma liposome transfection |
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