| 炭疽杆菌水肿因子基因的克隆与序列测定 |
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| 引用本文： | 袁斌,何君,王慧,荫俊.炭疽杆菌水肿因子基因的克隆与序列测定[J].生物技术通讯,2000,11(4):249-251. |
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| 作者姓名： | 袁斌 何君 王慧 荫俊 |
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| 作者单位： | 军事医学科学院微生物流行病研究所,北京,100071 |
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| 摘 要： | 采用聚合酶链反应（ＰＣＲ）从炭疽芽孢杆菌减毒株ＹＢ１中扩增其水肿因子（ＥＦ）的编码区基因，将其克隆至ｐＧＥＭ－Ｔ载体中，并分步测定其序列。序列测定表明，该基因长２ ３０１ ｂｐ，编码７６７个氨基酸，与已报道的Ｓｔｅｒｎｅ标准株的ＥＦ基因完全一致。
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| 关 键 词： | 炭疽杆菌 水肿因子 基因克隆 序列分析 |
Cloning and sequencing of the Bacillus anthracis edema factor gene |
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| Yuan Bin,He Jun,Wang Hui,Yin Jun.Cloning and sequencing of the Bacillus anthracis edema factor gene[J].Letters in Biotechnology,2000,11(4):249-251. |
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| Authors: | Yuan Bin He Jun Wang Hui Yin Jun |
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| Abstract: | The gene encoding edema gene (EF) of Bacillus anthracis was amplified from the attenuated strain YB1 by PCR, then cloned into pGEM?T vector and sequenced by step. The gene was 2301 bp long and encoding 767 amino acids, and it was the same as published EF gene of the standard Sterne strain. |
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| Keywords: | Bacillus anthracis edema factor cloning sequencing |
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