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| 酶法转化DL-ATC合成L-半胱氨酸的酶促反应条件研究 | |
| 引用本文： | 周昌平,怀丽华,白钢,杨文博,陈宁.酶法转化DL-ATC合成L-半胱氨酸的酶促反应条件研究[J].生物技术通讯,2006,17(6):911-913. |
| 作者姓名： | 周昌平 怀丽华 白钢 杨文博 陈宁 |
| 作者单位： | 1. 天津市工业微生物重点实验室,天津,300457 2. 天津市工业微生物重点实验室,天津,300457;天津商学院,天津,300134 3. 南开大学,生命科学学院,天津,300071 |
| 基金项目： | 天津市科委应用基础研究重点基金项目(05YFJZJC00901) |
| 摘 要： | 目的:考察酶源保存方式、酶促反应时间、底物pH值、底物浓度、酶浓度、金属离子等因素对酶活力的影响。方法:以假单胞菌(Pseudomonassp.)TS1138为供试菌株,采用酸式茚三酮法测定L-半胱氨酸含量,研究了酶法转化DL-ATC合成L-半胱氨酸的酶促反应条件。结果:TS1138菌株中L-半胱氨酸脱巯基酶具有较高的活性,而且Mg2 、Mn2 、Fe2 、Zn2 、Cu2 等5种金属离子对DL-ATC水解酶酶系有不同程度的抑制,其中Cu2 对该酶系的抑制作用很大。结论:确定了TS1138菌株酶法转化DL-ATC合成L-半胱氨酸的最适酶促反应条件,为酶促反应动力学的研究奠定了基础。 |
| 关 键 词： | 假单胞菌 酶促反应 L-半胱氨酸 |
| 文章编号： | 1009-0002(2006)06-0911-03 |
| 收稿时间： | 05 26 2006 12:00AM |
| 修稿时间： | 2006年5月26日 |
Study on Enzymatic Reaction Conditions of Conversion of DL-ATC to L-cysteine |
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| ZHOU Chang-ping,HUAI Li-hua,BAI Gang,YANG Wen-bo,CHEN Ning.Study on Enzymatic Reaction Conditions of Conversion of DL-ATC to L-cysteine[J].Letters in Biotechnology,2006,17(6):911-913. | |
| Authors: | ZHOU Chang-ping HUAI Li-hua BAI Gang YANG Wen-bo CHEN Ning |
| Institution: | 1. Tianjin Key Lab of Industrial Microbiology, Tianjin 300457; 2. Tianjin University of Commerce, Tianjin 300134; 3. College of Life Science, Nankai University, Tianjin 300017; China |
| Abstract: | Objective: To examine the effect of measures for conservation of enzyme, reaction time, pH, substrate concentration, enzyme concentration and metal ion on enzyme activity. Methods: Pseudomonas sp. TS1138 was used as the test strain to produce L-cysteine. Concentration of L-cysteine was measured by acidic ninhydrin reaction. Enzymatic reaction of conversion of DL-ATC to L-cysteine was studied. Results: L-cysteine desulfhydrase in Pseudomonas sp. TS1138 had high activity. Mg2 , Mn2 , Fe2 , Zn2 and Cu2 had different inhibition effects on the enzyme of conversion of DL-ATC to L-cysteine. Moreover, Cu2 had strong inhibition effects on the enzyme. Conclusion: Enzymatic reaction conditions of conversion of DL-ATC to L-cysteine are confirmed. |
| Keywords: | Pseudomonas sp enzymatic reaction L-cysteine |
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