| 双向凝胶电泳缺陷胶及其原因 |
| |
| 引用本文： | 王鸿丽,刘锋,赵永强,李萍,杨松成,魏开华.双向凝胶电泳缺陷胶及其原因[J].生物技术通讯,2008,19(4):569-571. |
| |
| 作者姓名： | 王鸿丽 刘锋 赵永强 李萍 杨松成 魏开华 |
| |
| 作者单位： | 国家生物医学分析中心,北京,100850 |
| |
| 摘 要： | 双向凝胶电泳是蛋白质组学研究中的关键技术之一,涉及较多的实验步骤和试剂,常出现缺陷胶而导致实验失败。从数千张电泳胶图中选取了21张典型的缺陷胶图,对它们进行了归类和总结,分析和讨论了形成缺陷胶的多种原因,提出了实验改进的方法和注意事项。
|
| 关 键 词： | 双向电泳 缺陷胶 蛋白质组学 |
Analysis of Defect Gels in 2D Gel Electrophoresis |
| |
| WANG Hong-Li,LIU Feng,ZHAO Yong-Qiang,LI Ping,YANG Song-Cheng,WEI Kai-Hua.Analysis of Defect Gels in 2D Gel Electrophoresis[J].Letters in Biotechnology,2008,19(4):569-571. |
| |
| Authors: | WANG Hong-Li LIU Feng ZHAO Yong-Qiang LI Ping YANG Song-Cheng WEI Kai-Hua |
| |
| Institution: | (National Center of Biomedical Analysis,Beijing 100850,China) |
| |
| Abstract: | Two-dimensional gel electrophoresis（2DE） is a key technology for proteomics,however,it often ended in defect gels in real experiments.Mistakes are easy to make because so many steps and materials are involved in operation.Twenty-one pictures of typical defect gels were chosen and classified,possible reasons were discussed,and some methods were proposed here. |
| |
| Keywords: | two-dimensional gel electrophoresis defect gel proteomics |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
