| 麦冬根中总RNA的快速提取 |
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| 引用本文： | 张俊,孙鹏,周丽莉,祁建军,李先恩.麦冬根中总RNA的快速提取[J].生物技术通讯,2010,21(2):255-257. |
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| 作者姓名： | 张俊 孙鹏 周丽莉 祁建军 李先恩 |
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| 作者单位： | 中国医学科学院,药用植物研究所,北京,100193 |
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| 摘 要： | 目的:从富含多糖、多酚的麦冬根部组织中快速提取总RNA。方法:采用改进的苯酚法,提取液的配制为5%SDS、1mol/LNaAc(pH4.1)、20%HAC、0.1%PVP。结果:采用该方法提取的麦冬总RNA纯度高、完整性好,电泳条带清晰。通过琼脂糖凝胶电泳与紫外吸光度测定产量与纯度,麦冬根部组织总RNA的吸光值D260nm/D280nm值大于1.8,D260nm/D230nm值大于2.0,麦冬块根与不定根RNA的平均产量分别为79.716和76.144μg/g(鲜重)。结论:用本方法提取的RNA可用于后继的抑制消减杂交试验。
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| 关 键 词： | 麦冬 RNA提取 多糖 多酚 抑制消减杂交 |
Quick Extraction of Total RNA from the Root of Ophiopogon japonicus (L.F.) Ker-Gawl |
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| ZHANG Jun,SUN Peng,ZHOU Li-Li,QI Jian-Jun,LI Xian-En.Quick Extraction of Total RNA from the Root of Ophiopogon japonicus (L.F.) Ker-Gawl[J].Letters in Biotechnology,2010,21(2):255-257. |
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| Authors: | ZHANG Jun SUN Peng ZHOU Li-Li QI Jian-Jun LI Xian-En |
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| Institution: | ZHANG Jun,SUN Peng,ZHOU Li-Li,QI Jian-Jun,LI Xian-En Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Beijing 100193,China |
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| Abstract: | Objective:To develop an effective method for total RNA isolation from the root tissue of Ophiopogon japonicus(L.F.) Ker-Gawl rich in polysaccharides and polyphenolics.Methods:Improved phenol method was applied.RNA extraction buffer was composed of 5% SDS,1 mol /L NaAc(pH4.1),20% HAC,and 0.1% PVP.Results:The RNA extraction protocol presented might be used to efficiently isolate high quality RNA with high purity and integrity,clear electrophoresis.Yield and purity were monitored by gel electrophoresis and by ... |
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| Keywords: | Ophiopogon japonicus RNA extraction polysaccharides polyphenolics SSH |
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