| 鼠疫耶尔森氏菌LcrV基因的克隆及序列分析 |
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| 引用本文： | 王忠泽,荫俊,王威,白洁,侯晓军,宋伟,张松乐.鼠疫耶尔森氏菌LcrV基因的克隆及序列分析[J].生物技术通讯,2001,12(1):18-19. |
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| 作者姓名： | 王忠泽 荫俊 王威 白洁 侯晓军 宋伟 张松乐 |
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| 作者单位： | 1. 北京微生物流行病研究所
2. 白求恩医科大学 |
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| 摘 要： | 为了研究鼠疫耶尔森氏菌（Y.pestis）保护性抗原V蛋白，从基因库中查得Y.pestis LcrV基因DNA序列，针对序列设计合成了一对PCR扩增引物，以本所保存的Y.pestis菌种为模板进行基因扩增，结果获得长约980bp的DNA片段。将扩增产物回收纯化，克隆至pGEM-T载体，构建重组载体pGEN-T/ypV，经过PCR，酶切鉴定，并对pGEM-T/ypV中的V基因片段进行测序，分析测序结果与己知序列相同，表明获得了LcrV基因。
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| 关 键 词： | 鼠疫耶尔森氏菌 基因克隆 测序 LcrV基因 基因克隆 |
| 修稿时间： | 2000年8月4日 |
Cloning and sequencing of
LcrVgene of Yersinia pestis |
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| WANG ZhongZe,YIN Jun,WANG Wei,BAI Jie,HO U XiaoJun,SOUNG Wei,ZHANG Songle.Cloning and sequencing of
LcrVgene of Yersinia pestis[J].Letters in Biotechnology,2001,12(1):18-19. |
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| Authors: | WANG ZhongZe YIN Jun WANG Wei BAI Jie HO U XiaoJun SOUNG Wei ZHANG Songle |
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| Institution: | WANG Zhong?Ze 1,YIN Jun 1,WANG Wei 1,BAI Jie 2,HOU Xiao?Jun 1,SOUNG Wei 1,ZHANG Song?Le 1 |
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| Abstract: | To study the protective antigen V protein of Yersinia pestis, a pair of primers for PCR were designed according to published DNA sequence of Y. pestis LcrV gene and synthesized. A DNA fragment about 980 bp length was gotten through PCR amplification. After recycled and purified, the DNA fragment was ligated with pGEM?T Vector and recombined vector pGEM?T/ypV was constructed. The pGEM?T/ypV plasmid was then identified by PCR, enzyme digestion and sequencing, and the sequence was the same as the published sequence. |
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| Keywords: | Yersinia pestis gene clone sequencing |
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