| CUEDC2的N端结构域溶液结构初步探索 |
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| 引用本文： | 张明,崔佳梅,王晨辉,张学敏,颜贤忠.CUEDC2的N端结构域溶液结构初步探索[J].生物技术通讯,2010,21(5):610-613. |
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| 作者姓名： | 张明 崔佳梅 王晨辉 张学敏 颜贤忠 |
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| 作者单位： | 军事医学科学院,生物医学分析中心,北京,100850 |
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| 摘 要： | 目的:为了更好地研究CUEDC2(CUE domain containing 2)的生物学功能,对其N端含133个氨基酸残基的结构域的三维结构进行初步探索。方法:用亲和纯化方法纯化GST-CUEDC2(1-133 aa)和His-CUEDC2(1-133aa),采用圆二色谱和核磁共振方法初步研究其结构。结果:圆二色谱实验表明CUEDC2(1-133 aa)cut比CUEDC2(1-133 aa)uncut的螺旋结构更多,折叠更好;核磁共振实验表明CUEDC2(1-133 aa)cut的1H-15N HSQC谱交叉峰更多,峰强度更均匀。结论:CUEDC2(1-133 aa)cut为最适合用核磁共振方法进行结构计算的片段。
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| 关 键 词： | CUEDC2 N端结构域 纯化 核磁共振 结构生物学 |
Preliminary Exploration of the Solution Structure of CUEDC2 N-Terminal Domain |
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| ZHANG Ming,CUI Jia-Mei,WANG Chen-Hui,ZHANG Xue-Min,YAN Xian-Zhong.Preliminary Exploration of the Solution Structure of CUEDC2 N-Terminal Domain[J].Letters in Biotechnology,2010,21(5):610-613. |
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| Authors: | ZHANG Ming CUI Jia-Mei WANG Chen-Hui ZHANG Xue-Min YAN Xian-Zhong |
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| Institution: | National Center of Biomedical Analysis,Academy of Military Medical Sciences,Beijing 100850,China |
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| Abstract: | Objective： To facilitate the functional studies of CUEDC2（CUE domain containing 2）,we decided to solve the N-terminal domain solution structure of CUEDC2.Methods： We purified GST-CUEDC2（1-133 aa） and His-CUEDC2（1-133 aa） with affinity purifying technology,then analyzed it with circular dichroism（CD） and nuclear magnetic resonance（NMR）.Results： The CD data showed CUEDC2（1-133 aa）cut had more helix then CUEDC2（1-133 aa）uncut;and 1H-15N HSQC NMR spectroscopy showed that the peaks of CUEDC2（1-133 aa）cut were more in quantity and more uniformity in strength.Conclusion： The results showed that CUEDC2（1-133 aa）cut was more suitable for solution structure study. |
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| Keywords: | CUEDC2 |
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