| 大鼠丝氨酸或半胱氨酸蛋白酶抑制剂B2的表达、纯化及抗体制备 |
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| 引用本文： | 马祖兴,高宁,于芳,林宇翔,绳纪坡,胡宝成.大鼠丝氨酸或半胱氨酸蛋白酶抑制剂B2的表达、纯化及抗体制备[J].生物技术通讯,2011,22(1):1-5. |
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| 作者姓名： | 马祖兴 高宁 于芳 林宇翔 绳纪坡 胡宝成 |
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| 作者单位： | 1. 军事医学科学院生物工程研究所,北京,100850;福建农林大学生命科学院,福建,福州,350002
2. 军事医学科学院生物工程研究所,北京,100850 |
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| 基金项目： | 国家自然科学基金，国家"十一五"支撑项目，国家重点基础研究发展计划 |
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| 摘 要： | 目的:原核表达、纯化大鼠丝氨酸或半胱氨酸蛋白酶抑制剂B2(SERPINB2),并制备其多克隆抗体.方法:设计扩增大鼠Serpinb2全长基因的特异引物,通过PCR扩增出该基因片段,测序正确后插入含GST基因的原核表达载体pGEX-KG中,以IPTG诱导表达,并经谷胱甘肽琼脂糖珠纯化融合蛋白;用纯化的蛋白免疫小鼠制备多克...
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| 关 键 词： | 丝氨酸或半胱氨酸蛋白酶抑制剂B2 表达 纯化 多克隆抗体 |
Expression and Purification of SERPINB2 and Preparation of its Polyclonal Antibody |
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| MA Zu-Xing,GAO Ning,YU Fang,LIN Yu-Xiang,SHENG Ji-Po,HU Bao-Cheng.Expression and Purification of SERPINB2 and Preparation of its Polyclonal Antibody[J].Letters in Biotechnology,2011,22(1):1-5. |
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| Authors: | MA Zu-Xing GAO Ning YU Fang LIN Yu-Xiang SHENG Ji-Po HU Bao-Cheng |
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| Institution: | MA Zu-Xing1,2,GAO Ning1,YU Fang1,LIN Yu-Xiang1,SHENG Ji-Po1,HU Bao-Cheng1 1.Beijing Institute of Biotechnology,Beijing 100850,2.School of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,China |
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| Abstract: | Objective:To express and purify serine(or cysteine) proteinase inhibitor B2(SERPINB2) in E.coli,and prepare the polyclonal antibody against SERPINB2.Methods:The specific primers were used to amplify full length of Serpinb2 gene by PCR,then the gene was inserted into the vector pGEX-KG containing GST gene,GST-SERPINB2 fusion protein was expressed in E.coli,and purified by using Glutathione Sepharose 4B.Then anti-SERPINB2 antiserum was prepared in mice,the titer was determined by ELISA and the specificity was... |
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| Keywords: | serine(or cysteine) proteinase inhibitor B2 expression purification polyclonal antibody |
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